Version 1
: Received: 21 August 2023 / Approved: 21 August 2023 / Online: 22 August 2023 (02:51:58 CEST)
Version 2
: Received: 29 August 2023 / Approved: 29 August 2023 / Online: 30 August 2023 (02:33:14 CEST)
How to cite:
Phan, M.; Nguyen-Thi, B.; Dinh, V.; Nguyen, T. Low-Level Laser Therapy Promotes Wound Healing in Fibroblasts Isolated from Human Chronic Wounds. Preprints2023, 2023081448. https://doi.org/10.20944/preprints202308.1448.v1
Phan, M.; Nguyen-Thi, B.; Dinh, V.; Nguyen, T. Low-Level Laser Therapy Promotes Wound Healing in Fibroblasts Isolated from Human Chronic Wounds. Preprints 2023, 2023081448. https://doi.org/10.20944/preprints202308.1448.v1
Phan, M.; Nguyen-Thi, B.; Dinh, V.; Nguyen, T. Low-Level Laser Therapy Promotes Wound Healing in Fibroblasts Isolated from Human Chronic Wounds. Preprints2023, 2023081448. https://doi.org/10.20944/preprints202308.1448.v1
APA Style
Phan, M., Nguyen-Thi, B., Dinh, V., & Nguyen, T. (2023). Low-Level Laser Therapy Promotes Wound Healing in Fibroblasts Isolated from Human Chronic Wounds. Preprints. https://doi.org/10.20944/preprints202308.1448.v1
Chicago/Turabian Style
Phan, M., Van-Han Dinh and Thi-Huong Nguyen. 2023 "Low-Level Laser Therapy Promotes Wound Healing in Fibroblasts Isolated from Human Chronic Wounds" Preprints. https://doi.org/10.20944/preprints202308.1448.v1
Abstract
This study aimed to evaluate the proliferation and migration of fibroblast cells derived from chronic wounds and investigate the impact of low-level laser therapy (LLLT) on their growth and migration. A total of 60 dermal samples were obtained from three sites of chronic pressure ulcers and diabetic ulcers in 20 patients. Following collection in the operating room, the dermal samples were isolated using the procedure described by Freshney RI. Fibroblasts from the wound base (Site 1) exhibited a slow proliferation rate and a decrease in proliferative capacity. Fibroblasts from wound margins (Site 2) and adjacent healing skin (Site 3) could be successfully isolated and maintained for multiple generations without any morphological changes. Fibroblasts from chronic wounds at Sites 2 and 3 showed slower proliferation rates and reduced migration capabilities compared to normal dermal fibroblasts (control samples). The fibroblast cells were exposed to LLLT at different energy levels (2.5, 3, and 3.5 J/cm2). The laser-treated groups demonstrated a significantly higher increase in cell count compared to the control sample, with the highest increase observed at an energy level of 3 J/cm2. These findings suggest that LLLT at an energy level of 3 J/cm2 could effectively enhance fibroblast migration, indicating its potential as a therapeutic intervention for chronic wounds.
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
