Teksoy, N.; Ilktac, M.; Ongen, B. Investigating the Significance of Non-jejuni/coli Campylobacter Strains in Patients with Diarrhea. Healthcare2023, 11, 2562.
Teksoy, N.; Ilktac, M.; Ongen, B. Investigating the Significance of Non-jejuni/coli Campylobacter Strains in Patients with Diarrhea. Healthcare 2023, 11, 2562.
Teksoy, N.; Ilktac, M.; Ongen, B. Investigating the Significance of Non-jejuni/coli Campylobacter Strains in Patients with Diarrhea. Healthcare2023, 11, 2562.
Teksoy, N.; Ilktac, M.; Ongen, B. Investigating the Significance of Non-jejuni/coli Campylobacter Strains in Patients with Diarrhea. Healthcare 2023, 11, 2562.
Abstract
Campylobacter is one of the most commonly reported foodborne bacteria worldwide. Although C. jejuni and C. coli have been reported to be responsible for the great majority of campylobacterio-sis, the burden of infections by species other than C. jejuni and C. coli have been increasing as a result of a transition to diagnostic test methods that enable the isolation of emerging species. The aim of the present study was to recover C. jejuni, C. coli, and emerging species from the stool samples of 500 patients with gastroenteritis and 100 healthy subjects by use of a filtration meth-od and culture techniques using Butzler agar and mCCDA under microaerophilic or hydrogen enriched atmosphere, identify the species by multiplex PCR methods and assess the significance of emerging species in enteric diseases. Thirty-one (6.2%) Campylobacter spp. were isolated from the stool samples of diarrheic patients but none from healthy individuals. Of 31 isolates, 21 (67.8%), 9 (29%) and 1 (3.2%) were identified as C. jejuni, C. coli and C. concisus by multiplex PCR, respectively. Filtration method was superior to the culture technique using mCCDA under mi-croaerophilic atmosphere. C. concisus was evaluated as the etiology of gastroenteritis as a result of laboratory and clinical evaluations. The present study was the first to indicate that emerging Campylobacter species are rarely detected and C. concisus is linked to acute gastroenteritis in Tur-key where additional studies are warranted to clarify the significance of emerging species in gastroenteritis.
Keywords
Campylobacter spp.; C. concisus; gastroenteritis; multiplex PCR; non-jejuni/coli
Subject
Medicine and Pharmacology, Epidemiology and Infectious Diseases
Copyright:
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