Li, Y.; Wang, C.; Cui, H.; Zhu, K.; Jia, F.; Ma, C.; Kang, J.; Sun, Y. Identification and Characterization of Alfalfa lncRNAs Based on PacBio Sequencing. Preprints2023, 2023081048. https://doi.org/10.20944/preprints202308.1048.v1
APA Style
Li, Y., Wang, C., Cui, H., Zhu, K., Jia, F., Ma, C., Kang, J., & Sun, Y. (2023). Identification and Characterization of Alfalfa lncRNAs Based on PacBio Sequencing. Preprints. https://doi.org/10.20944/preprints202308.1048.v1
Chicago/Turabian Style
Li, Y., Junmei Kang and Yan Sun. 2023 "Identification and Characterization of Alfalfa lncRNAs Based on PacBio Sequencing" Preprints. https://doi.org/10.20944/preprints202308.1048.v1
Abstract
Alfalfa is an important forage crop around the world. LncRNAs are considered to be a class of functional biomacromolecules, while little is known about lncRNAs in alfalfa. In this study, RNAs from different tissues of alfalfa were sequenced and anlyzed with full-length transcriptome sequencing technology, yielding the full-length transcripts dataset of alfalfa. Based on sequencing and public RNA-seq data, lncRNAs of alfalfa were predicted genome-wide by CPC2 and PLEK. The results showed most lncRNAs shared low sequence conservation with those in other plant species, part of which seems originate from plastid genome. We also identified 88563 lncRNAs, approximately 99.8% of total lncRNAs, with possibility of coding small ORFs using two prediction tools. Our research generated the biggest sequence set of alfalfa lncRNAs, and revealed some plastid originated lncRNAs with high sequence conservation.
Keywords
lncRNA; alfalfa; full-length transcriptome sequencing; sequence conservation; small ORF
Subject
Biology and Life Sciences, Plant Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
