Version 1
: Received: 12 August 2023 / Approved: 14 August 2023 / Online: 14 August 2023 (11:19:30 CEST)
Version 2
: Received: 5 October 2023 / Approved: 6 October 2023 / Online: 6 October 2023 (08:32:17 CEST)
Patlataya, N.N.; Bolshakov, I.N.; Levenets, A.A.; Medvedeva, N.N.; Khorzhevskii, V.A.; Cherkashina, M.A. Experimental Early Stimulation of Bone Tissue Neo-Formation for Critical Size Elimination Defects in the Maxillofacial Region. Polymers2023, 15, 4232.
Patlataya, N.N.; Bolshakov, I.N.; Levenets, A.A.; Medvedeva, N.N.; Khorzhevskii, V.A.; Cherkashina, M.A. Experimental Early Stimulation of Bone Tissue Neo-Formation for Critical Size Elimination Defects in the Maxillofacial Region. Polymers 2023, 15, 4232.
Patlataya, N.N.; Bolshakov, I.N.; Levenets, A.A.; Medvedeva, N.N.; Khorzhevskii, V.A.; Cherkashina, M.A. Experimental Early Stimulation of Bone Tissue Neo-Formation for Critical Size Elimination Defects in the Maxillofacial Region. Polymers2023, 15, 4232.
Patlataya, N.N.; Bolshakov, I.N.; Levenets, A.A.; Medvedeva, N.N.; Khorzhevskii, V.A.; Cherkashina, M.A. Experimental Early Stimulation of Bone Tissue Neo-Formation for Critical Size Elimination Defects in the Maxillofacial Region. Polymers 2023, 15, 4232.
Abstract
Rationale. A biomaterial is proposed for closing extensive bone defects in the maxillofacial region. The composition of the biomaterial includes high molecular weight chitosan, chondroitin sulfate, hyaluronate, heparin, alginate and inorganic nanostructured hydroxyapatite. The purpose of the study is to demonstrate morphological and histological early signs of reconstruction of a bone cavity of a critical size. Materials and methods. The studies were carried out on 84 white female rats weighing 200 g. The control group of animals consisted of 44 individuals, the study group - 40. In all animals, three-walled bone defects 0.5 cm in size were applied subperiosteally in the region of the angle of the lower jaw and filled in the experimental group with a gel mass of chitosan -alginate-hydroxyapatite (CH-SA-HA). In control animals, the bone cavities were filled with an auto-blood clot. The follow-up periods were 3.5.7 days, 2.3.4.6.8 and 10 weeks. Results. The chitosan construct after implantation actively replaced defects early with the formation of a full-fledged new bone tissue compared to control animals. Morphological analysis of the walls of the bone defect on the 7th day showed signs of the formation of spongy bone tissue, after two weeks - a pronounced increase in bone volume (P<0.01), after 6 weeks after surgery, the closure of the defect was 70-80%, after 8 weeks - 100% without disturbing bone morphology with a high degree of mineralization. Conclusion The use of modified chitosan after filling eliminates bone defects of critical size in the maxillofacial region, reveals early signs of bone neoformation, and serves as a promising material in reconstructive dentistry.
Keywords
experimental critical size bone defect; maxillofacial area; modified chitosan; early bone formation; morphometry; rats
Subject
Medicine and Pharmacology, Dentistry and Oral Surgery
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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