PreprintHypothesisVersion 1Preserved in Portico This version is not peer-reviewed
Quantifying Tissue Response after Intraovarian Platelet-Rich Plasma: Experimental Approach to Assess NF-κB and TNF-α as Mediators to Reduce Local Entropy
Version 1
: Received: 13 August 2023 / Approved: 14 August 2023 / Online: 14 August 2023 (10:31:12 CEST)
How to cite:
Sills, E.S. Quantifying Tissue Response after Intraovarian Platelet-Rich Plasma: Experimental Approach to Assess NF-κB and TNF-α as Mediators to Reduce Local Entropy. Preprints2023, 2023081004. https://doi.org/10.20944/preprints202308.1004.v1
Sills, E.S. Quantifying Tissue Response after Intraovarian Platelet-Rich Plasma: Experimental Approach to Assess NF-κB and TNF-α as Mediators to Reduce Local Entropy. Preprints 2023, 2023081004. https://doi.org/10.20944/preprints202308.1004.v1
Sills, E.S. Quantifying Tissue Response after Intraovarian Platelet-Rich Plasma: Experimental Approach to Assess NF-κB and TNF-α as Mediators to Reduce Local Entropy. Preprints2023, 2023081004. https://doi.org/10.20944/preprints202308.1004.v1
APA Style
Sills, E.S. (2023). Quantifying Tissue Response after Intraovarian Platelet-Rich Plasma: Experimental Approach to Assess NF-κB and TNF-α as Mediators to Reduce Local Entropy. Preprints. https://doi.org/10.20944/preprints202308.1004.v1
Chicago/Turabian Style
Sills, E.S. 2023 "Quantifying Tissue Response after Intraovarian Platelet-Rich Plasma: Experimental Approach to Assess NF-κB and TNF-α as Mediators to Reduce Local Entropy" Preprints. https://doi.org/10.20944/preprints202308.1004.v1
Abstract
Diminished ovarian reserve can be regarded as a sentinel indicator to foreshadow severe follicular loss and, ultimately, systemic aging. The negative slope of human ovulatory fidelity begins with a robust follicular endowment which gradually declines over time. In contrast, the youthful ovarian phenotype requires the coordinated work of endothelial, granulosa, immune, perivascular, stromal and possibly germline stem cells. Such a diverse tissue matrix can, in general, be influenced by platelet (PLT)-derived factors but this has not yet been specifically confirmed in the ovary after platelet-rich plasma (PRP). How could a comparable response be validated? Here a prospective, experimental study is proposed whereby eligible patients already undergoing scheduled laparoscopy provide ovarian tissue via biopsy submitted for co-culture with autologous Ca+2 activated PRP. Recognizing the interlocking, central roles of nuclear factor κB (NF-κB) and tumor necrosis factor-α (TNF-α), incubated samples would be assessed for these in vitro before vs. after PRP exposure, in addition to stereomicroscopy. A mathematical model is available to track NF-κB oscillations and estimate gene expression, cell development, growth, apoptosis, and key immune and inflammatory actions. Since NF-κB and TNF-α are discharged in activated PLT releasate (or react to its cargo proteins) this audit permits extraction of response markers observed post-stimulus, thus linking discrete signals to transcriptional output, cellular fitness, and ovarian cytoarchitecture. From this, a hypothesis could emerge where intraovarian PRP is found to make no direct impact on follicles, although modified ovarian field function and curtailed local entropy incidentally favor optimized oocyte competence as a secondary effect.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
