Ma, X.; Chen, Y.; Shu, A.; Jiang, Y.; Chen, X.; Ma, C.; Zhou, M.; Wang, T.; Chen, T.; Shaw, C.; Wang, L. A Novel Antimicrobial Peptide, Dermaseptin-SS1, with Anti-Proliferative Activity, Isolated from the Skin Secretion of Phyllomedusa tarsius. Molecules2023, 28, 6558.
Ma, X.; Chen, Y.; Shu, A.; Jiang, Y.; Chen, X.; Ma, C.; Zhou, M.; Wang, T.; Chen, T.; Shaw, C.; Wang, L. A Novel Antimicrobial Peptide, Dermaseptin-SS1, with Anti-Proliferative Activity, Isolated from the Skin Secretion of Phyllomedusa tarsius. Molecules 2023, 28, 6558.
Ma, X.; Chen, Y.; Shu, A.; Jiang, Y.; Chen, X.; Ma, C.; Zhou, M.; Wang, T.; Chen, T.; Shaw, C.; Wang, L. A Novel Antimicrobial Peptide, Dermaseptin-SS1, with Anti-Proliferative Activity, Isolated from the Skin Secretion of Phyllomedusa tarsius. Molecules2023, 28, 6558.
Ma, X.; Chen, Y.; Shu, A.; Jiang, Y.; Chen, X.; Ma, C.; Zhou, M.; Wang, T.; Chen, T.; Shaw, C.; Wang, L. A Novel Antimicrobial Peptide, Dermaseptin-SS1, with Anti-Proliferative Activity, Isolated from the Skin Secretion of Phyllomedusa tarsius. Molecules 2023, 28, 6558.
Abstract
The emergence of multi-drug resistant bacteria has severely increased the burden on the global health system, and such pathogen infections are considered a great threat to human well-being. Antimicrobial peptides, due to their potent antimicrobial activity and low possibility of inducing resistance, are increasingly becoming the focus of much interest. Herein, a novel dermaseptin peptide, named Dermaseptin-SS1 (SS1), was identified from a skin secretion-derived cDNA library of the South/Central American tarsier leaf frog, Phyllomedusa tarsius, using a ‘shotgun’ cloning strategy. Chemically-synthesised peptide SS1 was found to be broadly effective against Gram-negative bacteria with low haemolytic activity in vitro. A designed synthetic analogue of SS1, named peptide 14V5K, showed a lower salt sensitivity with more rapid bacteria-killing compared to SS1. Both peptides employed the membrane-targeting mechanism to kill Escherichia coli. The antiproliferative activity of SS1 and its analogues, against lung cancer cell lines, was found to be significant.
Biology and Life Sciences, Biology and Biotechnology
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