Ouchida, T., Suzuki, H., Tanaka, T., Kaneko, M. K., & Kato, Y. (2024). Establishment of Anti-Dog Programmed Cell Death Ligand 1 Monoclonal Antibodies for Immunohistochemistry. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy.
Ouchida, T., Suzuki, H., Tanaka, T., Kaneko, M. K., & Kato, Y. (2024). Establishment of Anti-Dog Programmed Cell Death Ligand 1 Monoclonal Antibodies for Immunohistochemistry. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy.
Ouchida, T., Suzuki, H., Tanaka, T., Kaneko, M. K., & Kato, Y. (2024). Establishment of Anti-Dog Programmed Cell Death Ligand 1 Monoclonal Antibodies for Immunohistochemistry. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy.
Ouchida, T., Suzuki, H., Tanaka, T., Kaneko, M. K., & Kato, Y. (2024). Establishment of Anti-Dog Programmed Cell Death Ligand 1 Monoclonal Antibodies for Immunohistochemistry. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy.
Abstract
Immune checkpoint blockade therapy has shown successful clinical outcomes in multiple human cancers. In dogs, several types of tumors resemble human tumors in many respects. Therefore, several groups have developed the anti-dog programmed cell death ligand 1 (dPD-L1) monoclonal antibodies (mAbs) and showed efficacy in several canine tumors. To examine the abundance of dPD-L1 in canine tumors, anti-dPD-L1 diagnostic mAbs for immunohistochemistry are required. In this study, we immunized the peptide in the dPD-L1 intracellular domain, and established anti-dPD-L1 mAbs, L1Mab-352 (mouse IgG1, kappa) and L1Mab-354 (mouse IgG1, kappa). In enzyme-linked immunosorbent assay, L1Mab-352 and L1Mab-354 showed high binding affinity to the dPD-L1 peptide, and the dissociation constants (KD) were determined as 6.9 ×10-10 M and 7.2 ×10-10 M, respectively. Furthermore, L1Mab-352 and L1Mab-354 were applicable for the detection of dPD-L1 in immunohistochemical analysis in paraffin-embedded dPD-L1-expressed cells. These results indicated that L1Mab-352 and L1Mab-354 are useful for detecting dPD-L1 in immunohistochemical analysis.
Medicine and Pharmacology, Oncology and Oncogenics
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