Version 1
: Received: 1 August 2023 / Approved: 2 August 2023 / Online: 2 August 2023 (14:37:19 CEST)
How to cite:
Li, Y.; Zhang, J.; Liang, Z.; Zhang, R.; Wu, W. TCGA and Experimental Validation-Based Analysis of the SPRR2A Gene's Expression and Clinical Relevance in Endometrial Cancer. Preprints2023, 2023080237. https://doi.org/10.20944/preprints202308.0237.v1
Li, Y.; Zhang, J.; Liang, Z.; Zhang, R.; Wu, W. TCGA and Experimental Validation-Based Analysis of the SPRR2A Gene's Expression and Clinical Relevance in Endometrial Cancer. Preprints 2023, 2023080237. https://doi.org/10.20944/preprints202308.0237.v1
Li, Y.; Zhang, J.; Liang, Z.; Zhang, R.; Wu, W. TCGA and Experimental Validation-Based Analysis of the SPRR2A Gene's Expression and Clinical Relevance in Endometrial Cancer. Preprints2023, 2023080237. https://doi.org/10.20944/preprints202308.0237.v1
APA Style
Li, Y., Zhang, J., Liang, Z., Zhang, R., & Wu, W. (2023). TCGA and Experimental Validation-Based Analysis of the SPRR2A Gene's Expression and Clinical Relevance in Endometrial Cancer. Preprints. https://doi.org/10.20944/preprints202308.0237.v1
Chicago/Turabian Style
Li, Y., Ranran Zhang and Wenming Wu. 2023 "TCGA and Experimental Validation-Based Analysis of the SPRR2A Gene's Expression and Clinical Relevance in Endometrial Cancer" Preprints. https://doi.org/10.20944/preprints202308.0237.v1
Abstract
Objective: To verify SPRR2A is a hub gene for endometrial cancer (EC). Methods: Bioinformatics analysis was performed on the high-throughput sequencing dataset of 587 EC cases in TCGA. RT-qPCR verified the differential expression of the SPRR2A gene in EC cell lines. Results: Enrichment of differentially expressed genes in comparison with normal tissues has been found in several biological process pathways, including keratinization and epidermal growth. The signalling pathways of differentially expressed genes were mainly focused on the receptor-ligand pathway and passive transmembrane transport pathway. High SPRR2A expression was a poor prognostic indicator for EC, according to a one-way Cox analysis of TCGA EC data. Compared with normal tissues, EC tissues had considerably elevated SPRR2A expression according to the differential expressed genetic analysis of TCGA-UCEC data (P < 0.05). RT-qPCR was used to experimentally confirm that the Ishikawa EC cell line expressed SPRR2A mRNA at a greater level than the human endometrial epithelial cell line and that this difference was statistically obvious (P < 0.01). Conclusion: SPRR2A has been identified as hub gene for EC and potentially become a new marker for early diagnosis, precise treatment and prognosis predictor of EC.
Medicine and Pharmacology, Obstetrics and Gynaecology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
