Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Propylene Glycol Potentiates the Inhibitory Action of Ctz Paste on Antibiotic-Resistant Enterococcus faecalis Isolated from the Root Canal: An In Vitro Study

Jesús Yareli Rayos-Verdugo
,
Fernando Rivera-Chaparro
,
Gloria-Yolanda Castro-Salazar
,
Maricela Ramírez-Álvarez
,
José Geovanni Romero-Quintana
ORCID logo ,
Juan Pablo Loyola-Rodríguez
ORCID logo ,
Norma Verónica Zavala-Alonso
,
Mariana Avendaño-Félix
ORCID logo ,
Jesús Edurdo Soto-Sainz
,
Erika de Lourdes Silva-Benítez
* ORCID logo
Version 1 : Received: 1 August 2023 / Approved: 2 August 2023 / Online: 3 August 2023 (08:53:27 CEST)

A peer-reviewed article of this Preprint also exists.

Rayos-Verdugo, J.Y.; Rivera-Chaparro, F.; Castro-Salazar, G.Y.; Ramírez-Álvarez, M.; Romero-Quintana, J.G.; Loyola-Rodríguez, J.P.; Zavala-Alonso, N.V.; Avendaño-Félix, M.; Soto-Sainz, J.E.; Silva-Benítez, E.L. Propylene Glycol Potentiates the Inhibitory Action of CTZ Paste on Antibiotic-Resistant Enterococcus faecalis Isolated from the Root Canal: An In Vitro Study. Microorganisms 2023, 11, 2208. Rayos-Verdugo, J.Y.; Rivera-Chaparro, F.; Castro-Salazar, G.Y.; Ramírez-Álvarez, M.; Romero-Quintana, J.G.; Loyola-Rodríguez, J.P.; Zavala-Alonso, N.V.; Avendaño-Félix, M.; Soto-Sainz, J.E.; Silva-Benítez, E.L. Propylene Glycol Potentiates the Inhibitory Action of CTZ Paste on Antibiotic-Resistant Enterococcus faecalis Isolated from the Root Canal: An In Vitro Study. Microorganisms 2023, 11, 2208.

Abstract

This study aimed to evaluate if the change of vehicle for CTZ (chloramphenicol, tetracycline, zinc oxide, and eugenol) paste improves the inhibition of Enterococcus faecalis in vitro. The vehicles evaluated alone and mixed with CTZ were eugenol, propylene glycol (PG), chitosan, su-per-oxidized solution (SOS), grapefruit-seed extract (GSE), and as a negative control, 0.9% saline solution. A clinical isolated of E. faecalis was morphological and biochemical characterized, in-cluding antimicrobial susceptibility was tested using 20 antimicrobial agents. Once character-ized, the clinical isolated was cultivated to performed Kirby–Bauer disc diffusion method with paper discs embedded with the different vehicles mixed or used alone, incubating at 37 ºC for 24 h. Data were analyzed by One-way ANOVA and the means were compared using Tukey test with a significance level of p<0.05. For vehicles used alone, GSE presented the greatest inhibition showing a statistically significant difference with the rest of the vehicles. When vehicles were mixed with the CTZ paste, PG showed a greater inhibition with a statistically significant differ-ence from the rest of the vehicles. In conclusion, the vehicle used to mix the CTZ paste plays an im-portant role in the inhibition of E. faecalis in vitro, therefore we consider that this can be an im-portant factor to achieve success in the use of this technique.

Keywords

CTZ; grapefruit seed extract; propylene glycol; E. faecalis

Subject

Medicine and Pharmacology, Dentistry and Oral Surgery

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.