Version 1
: Received: 28 July 2023 / Approved: 28 July 2023 / Online: 31 July 2023 (03:19:47 CEST)
How to cite:
Wang, X.; An, H.; Ma, Y.; Chen, H.; Ke, S. Co-Analysis of the Metabolome and Transcriptome Reveals Regulation Network of Mint Pigment and Flavone. Preprints2023, 2023072025. https://doi.org/10.20944/preprints202307.2025.v1
Wang, X.; An, H.; Ma, Y.; Chen, H.; Ke, S. Co-Analysis of the Metabolome and Transcriptome Reveals Regulation Network of Mint Pigment and Flavone. Preprints 2023, 2023072025. https://doi.org/10.20944/preprints202307.2025.v1
Wang, X.; An, H.; Ma, Y.; Chen, H.; Ke, S. Co-Analysis of the Metabolome and Transcriptome Reveals Regulation Network of Mint Pigment and Flavone. Preprints2023, 2023072025. https://doi.org/10.20944/preprints202307.2025.v1
APA Style
Wang, X., An, H., Ma, Y., Chen, H., & Ke, S. (2023). Co-Analysis of the Metabolome and Transcriptome Reveals Regulation Network of Mint Pigment and Flavone. Preprints. https://doi.org/10.20944/preprints202307.2025.v1
Chicago/Turabian Style
Wang, X., Hong Chen and Shaoying Ke. 2023 "Co-Analysis of the Metabolome and Transcriptome Reveals Regulation Network of Mint Pigment and Flavone" Preprints. https://doi.org/10.20944/preprints202307.2025.v1
Abstract
Mint is considered to have a greater number of phenolic acids, flavonoids, antioxidants and other bioactive components, and is widely used as food, medicine, spices, and flavoring agents. Thus, chemical composition is an important parameter for assessing the quality of mint. In this study, two mint cultivars were sampled, purple mint and green mint. The purple mint had much higher anthocyanin and total flavone content compared with green mint. Transcriptome and metabolome technique were employed to elucidate the regulation network of mint pigment and flavone. A total of 167,901 unigenes were obtained by high-throughput RNA-Seq and 34,608 genes were differentially expressed. The differentially expressed genes (DEGs) were mainly involved in lignin metabolic process and flavonoid biosynthetic process. A total of 143 differentially expressed metabolites (DEMs) were enriched in isoflavonoid biosynthesis, flavonoid biosynthesis, flavone and flavonol biosynthesis, and anthocyanin biosynthesis pathways. The co-analysis results of DEGs and DEMs showed that the flavone and flavonol biosynthesis pathway (ko00944) contained the most DEMs, followed by flavonoid biosynthesis pathway (ko00941) and anthocyanin biosynthesis pathway (ko00942). Furthermore, the most important nine genes and metabolites were screened using O2PLS model. The results will provide a theoretical basis for revealing pigment and flavone biosynthesis network of mint.
Keywords
mint; pigment; flavone; transcriptome; metabolome
Subject
Biology and Life Sciences, Plant Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
