Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Gravisensitivity Of Stromal Collagen Fibers In The Gastrointestinal Hollow Organs Of Mice After A 30-Day Space Flight

Viktoriya Shishkina
ORCID logo ,
Andrey Kostin
,
Nataliya T Alexeeva
,
Svetlana V Klochkova
,
Dmitry B Nikityuk
,
Artem Volodkin
,
Igor Buchwalow
* ORCID logo ,
Markus Tiemann
,
Dmitri Atiakshin
ORCID logo
Version 1 : Received: 24 July 2023 / Approved: 24 July 2023 / Online: 25 July 2023 (08:10:23 CEST)

How to cite: Shishkina, V.; Kostin, A.; Alexeeva, N. T.; Klochkova, S. V.; Nikityuk, D. B.; Volodkin, A.; Buchwalow, I.; Tiemann, M.; Atiakshin, D. Gravisensitivity Of Stromal Collagen Fibers In The Gastrointestinal Hollow Organs Of Mice After A 30-Day Space Flight. Preprints 2023, 2023071661. https://doi.org/10.20944/preprints202307.1661.v1 Shishkina, V.; Kostin, A.; Alexeeva, N. T.; Klochkova, S. V.; Nikityuk, D. B.; Volodkin, A.; Buchwalow, I.; Tiemann, M.; Atiakshin, D. Gravisensitivity Of Stromal Collagen Fibers In The Gastrointestinal Hollow Organs Of Mice After A 30-Day Space Flight. Preprints 2023, 2023071661. https://doi.org/10.20944/preprints202307.1661.v1

Abstract

The digestive organs are highly sensitive to the influence of orbital flight factors and can limit the professional activities of crew members aboard the International Space Station. Connective tissue as a system-forming matrix of the integrative-buffer metabolic environment is of particular relevance in space biomedicine, ensuring the functioning of internal organs under an altered gravitational stimulus. However, the adaptive mechanisms of the fibrous extracellular matrix of the gastric and intestinal connective tissue have not been fully investigated under prolonged microgravity weightlessness. Using histochemical techniques, the authors experimentally studied the state of collagen fibers in the specific tissue microenvironment of the gastric and intestinal membranes in C57BL/6N mice after a 30-day space flight, subsequent 7-day ground readaptation, and in animals of the relevant control groups. The 30-day stay of laboratory animals aboard the Bion-M 1 biosatellite resulted in a reduction in the fibrous extracellular matrix of connective tissue in the studied digestive organs, excepting the gastric lamina propria. An increased fibrillogenesis was revealed in the gastrointestinal mucous membranes of animals in 7 days after the biosatellite landing, if compared with the parameters of animals of the space flight group. During the experiment with ground simulated orbital flight conditions, changes in collagen fibers were not significant compared to the vivarium control group. Thus, the results obtained evidence gravisensitivity of the fibrous extracellular matrix of the intraorgan connective tissue. This fact also highlights the necessity to further improve gastrointestinal tract-related preventive measures for astronauts during orbital flight.

Keywords

orbital flight; weightlessness; digestive system; connective tissue; collagen fibers; С57BL/6N mice

Subject

Biology and Life Sciences, Life Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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