Version 1
: Received: 12 July 2023 / Approved: 12 July 2023 / Online: 13 July 2023 (08:31:06 CEST)
How to cite:
Lutz, T.; Bien, S.; Langer, G.J.; Heinze, C. Transcapsidation and Polysomal Encapsulation as Putative Strategies for the Genome Protection of the Novel Diplodia fraxini Fusagravirus 1 (DfFV1). Preprints2023, 2023070901. https://doi.org/10.20944/preprints202307.0901.v1
Lutz, T.; Bien, S.; Langer, G.J.; Heinze, C. Transcapsidation and Polysomal Encapsulation as Putative Strategies for the Genome Protection of the Novel Diplodia fraxini Fusagravirus 1 (DfFV1). Preprints 2023, 2023070901. https://doi.org/10.20944/preprints202307.0901.v1
Lutz, T.; Bien, S.; Langer, G.J.; Heinze, C. Transcapsidation and Polysomal Encapsulation as Putative Strategies for the Genome Protection of the Novel Diplodia fraxini Fusagravirus 1 (DfFV1). Preprints2023, 2023070901. https://doi.org/10.20944/preprints202307.0901.v1
APA Style
Lutz, T., Bien, S., Langer, G.J., & Heinze, C. (2023). Transcapsidation and Polysomal Encapsulation as Putative Strategies for the Genome Protection of the Novel <em>Diplodia fraxini </em>Fusagravirus 1 (DfFV1). Preprints. https://doi.org/10.20944/preprints202307.0901.v1
Chicago/Turabian Style
Lutz, T., Gitta Jutta Langer and Cornelia Heinze. 2023 "Transcapsidation and Polysomal Encapsulation as Putative Strategies for the Genome Protection of the Novel <em>Diplodia fraxini </em>Fusagravirus 1 (DfFV1)" Preprints. https://doi.org/10.20944/preprints202307.0901.v1
Abstract
Two novel dsRNA mycoviruses were found in different isolates of Diplodia fraxini, NW FVA 1581 and NW FVA 1706, which were isolated from a root, associated with stem collar necrosis of Fraxinus excelsior L. Both mycelia are infected by a novel fusagravirus, which was named Diplodia fraxini fusagravirus 1 (DfFV1), and isolate NW FVA 1706 is additionally infected by a novel partitivirus, which was denominated as Diplodia fraxini partitivirus 1 (DfPV1). The one-segmented, bicistronic genome of DfFV1 is composed of about 8,500 bp. Their ORFs are connected by a 1 slippery heptamer sequence and the 3’ terminal ORF is coding for the viral RdRp. The genome of DfPV1 is composed of three, monocistronic dsRNA segments ranging from 1,755 bp (dsRNA 1) over 1,588 bp (dsRNA 2) to 1,233 bp (dsRNA 3). Based on genome organization and phylogenetic positions, DfFV1 was assigned to the proposed family of “Fusagraviridae” and DfPV1 to the genus Gammapartitivirus within the family of Partitiviridae.
Ultra-structural analysis showed that polysomal structures were stabilized in the single infection and none of these structures could be isolated in the double infection. It is assumed that DfFV1 has an opportunistic lifestyle, being either protected by ribosomes or by transcapsidation from particles of DfPV1.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
