Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Amino Acid Profiling of Follicular Fluid in Assisted Reproduction Reveals Important Roles of Several Amino Acids in Patients with Insulin Resistance

Csilla Kurdi
,
Vanessza Lelovics
ORCID logo ,
Dávid Hesszenberger
,
Anikó Lajtai
,
Ágnes Lakatos
,
Róbert Herczeg
,
Krisztina Gödöny
,
Péter Mauchart
,
Ákos Várnagy
,
Gábor L Kovács
ORCID logo ,
Tamás Kőszegi
*
Version 1 : Received: 11 July 2023 / Approved: 12 July 2023 / Online: 12 July 2023 (11:14:29 CEST)

A peer-reviewed article of this Preprint also exists.

Kurdi, C.; Lelovics, V.; Hesszenberger, D.; Lajtai, A.; Lakatos, Á.; Herczeg, R.; Gödöny, K.; Mauchart, P.; Várnagy, Á.; Kovács, G.L.; Kőszegi, T. Amino Acid Profiling of Follicular Fluid in Assisted Reproduction Reveals Important Roles of Several Amino Acids in Patients with Insulin Resistance. Int. J. Mol. Sci. 2023, 24, 12458. Kurdi, C.; Lelovics, V.; Hesszenberger, D.; Lajtai, A.; Lakatos, Á.; Herczeg, R.; Gödöny, K.; Mauchart, P.; Várnagy, Á.; Kovács, G.L.; Kőszegi, T. Amino Acid Profiling of Follicular Fluid in Assisted Reproduction Reveals Important Roles of Several Amino Acids in Patients with Insulin Resistance. Int. J. Mol. Sci. 2023, 24, 12458.

Abstract

The global prevalence of insulin resistance (IR) is increasing continuously, influencing metabolic parameters and fertility as well. The metabolic changes due to IR can alter the molecular composition of plasma and other body fluids. Follicular fluid (FF) is derived mainly from plasma, and it is a critical microenvironment for the developing oocytes. It contains various metabolites and amino acids, and the quality of the oocytes is linked at least partially to amino acid metabolism. Our goal was to determine quantitatively the amino acid (AA) profile of FF in IVF patients and to compare IR and non-insulin resistance (NIR) groups to investigate the AA changes in their FF. By UHPLC-based method we quantified the main 20 amino acids from human FF samples in the IR and NIR groups. Several amino acids (aspartate, glycine, glutamate, and cysteine) differed significantly (p<0.05 or less) between the two groups. The most significant alterations between the IR and NIR groups were related to the glutathione metabolic pathway involving glycine, serine, and threonine. Since insulin resistance alters the amino acid composition of the FF, the oocytes may undergo metabolism-induced changes resulting in poor oocyte quality and less fertility in the insulin resistance groups.

Keywords

Assisted reproduction technology; follicular fluid; metabolomics; insulin resistance; amino acid profile; predictive value

Subject

Medicine and Pharmacology, Endocrinology and Metabolism

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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