Huang, T.; Xing, Z.; Wu, S.; Duan, W.; Li, L.; Wang, Q.; Song, H.; Meng, L.; Xu, X. Silencing of miR171d/e in tomato enhances salt tolerance of plant and improves fruit nutritional quality. Preprints2023, 2023070733. https://doi.org/10.20944/preprints202307.0733.v1
APA Style
Huang, T., Xing, Z., Wu, S., Duan, W., Li, L., Wang, Q., Song, H., Meng, L., & Xu, X. (2023). Silencing of miR171d/e in tomato enhances salt tolerance of plant and improves fruit nutritional quality. Preprints. https://doi.org/10.20944/preprints202307.0733.v1
Chicago/Turabian Style
Huang, T., Lanhuan Meng and Xiangbin Xu. 2023 "Silencing of miR171d/e in tomato enhances salt tolerance of plant and improves fruit nutritional quality" Preprints. https://doi.org/10.20944/preprints202307.0733.v1
Abstract
High salinity in soil affects the normal growth and development of crop. MicroRNA171 (miR171) plays a momentous role in plant resistance to adversity stress. The functions of Sly-miR171d and Sly-miR171e on growth of tomato and fruit nutritional quality under salt stress were studied. The results showed that the seed germination and seedling growth of tomato that silencing of Sly-miR171d/e by short tandem target mimic (STTM) were better than those of wild-type (WT). Silencing of Sly-miR171d/e seeds maintained a high germination rate, while WT tomato seeds barely germinated under 50 mM NaCl stress. Under 100 mM NaCl stress, silencing of Sly-miR171d/e increased the expression of their target gene SlGRAS24 with increasing duration of salt stress, while they enhanced the antioxidant activity, reduced reactive oxygen species (ROS) accumulation, and enhanced the salt tolerance of tomato plant by regulating gibberellin (GA) level. Moreover, the preharvest and postharvest fruit nutritional quality under salt stress was also studied, and the results showed that silencing of Sly-miR171d/e increased soluble solids, lycopene, total carotenoids, Vitamin C, organic acids and phenolic substances in tomato fruit. The present study demonstrated that silencing of Sly-miR171d/e enhanced plant salt tolerance through the GA pathway, and improved fruit nutritional quality under salt stress.
Keywords
tomato; Sly-miR171d/e; short tandem target mimic (STTM); salt stress; fruit quality
Subject
Biology and Life Sciences, Plant Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
