Robea, M.A.; Petrovici, A.; Ureche, D.; Nicoara, M.; Ciobica, A.S. Histopathological and Behavioral Impairments in Zebrafish (Danio rerio) Chronically Exposed to a Cocktail of Fipronil and Pyriproxyfen. Life2023, 13, 1874.
Robea, M.A.; Petrovici, A.; Ureche, D.; Nicoara, M.; Ciobica, A.S. Histopathological and Behavioral Impairments in Zebrafish (Danio rerio) Chronically Exposed to a Cocktail of Fipronil and Pyriproxyfen. Life 2023, 13, 1874.
Robea, M.A.; Petrovici, A.; Ureche, D.; Nicoara, M.; Ciobica, A.S. Histopathological and Behavioral Impairments in Zebrafish (Danio rerio) Chronically Exposed to a Cocktail of Fipronil and Pyriproxyfen. Life2023, 13, 1874.
Robea, M.A.; Petrovici, A.; Ureche, D.; Nicoara, M.; Ciobica, A.S. Histopathological and Behavioral Impairments in Zebrafish (Danio rerio) Chronically Exposed to a Cocktail of Fipronil and Pyriproxyfen. Life 2023, 13, 1874.
Abstract
Background: Lately, the high incidence of pesticide usage has attracted everyone's interest due to the serious effects produced. Fipronil (FIP) is a phenylpyrazole compound that acts on the insect’s GABA neurotransmitter by inhibiting its activity. Moreover, literature reports highlight its impli-cation in neurodevelopmental abnormalities and oxidative stress production in different organisms. Similarly, pyriproxyfen (PYR) is known to affect insects’ activity by mimicking the natural hormones involved in the maturation of the young insects. The aim of the present study was to investigate the impact of these pesticides’ mixture on zebrafish tissues and behavior. Methods: To assess the in-fluence of this cocktail on zebrafish, three groups of animals were randomly selected and exposed to 0, 0.05 and 0.1 mg L-1 FIP and PYR mixture for 5 days. The fish were evaluated daily by the T-maze tests as locomotor activity and dark-light test and recorded for 4 minutes. The data was quantified using the EthoVision software. Results: Our results indicated significant changes in locomotor ac-tivity parameters showing increased levels following exposure to the mixture of FIP and PYR. On the other hand, the mixture also triggered anxiety in the zebrafish, which spent more time in the light area than in the dark. In addition, mixture-induced histological changes were observed in the form of numerous hemosiderin deposits found in various zebrafish tissues. Conclusions: The cur-rent findings indicate that the mixture of FIP and PYR can have considerable consequences on adult zebrafish and may promote or cause functional neurological changes in addition to histological ones.
Biology and Life Sciences, Ecology, Evolution, Behavior and Systematics
Copyright:
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