Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Comparative Genomic Analysis and Phenotypic Characterization of Saccharomyces cerevisiae Strains used in Sugarcane-based Fermentation Processes to Improve Sucrose Fermentation by an Industrial Fuel-ethanol Yeast Strain

Gabriela Muller
,
Victor R. De Godoy
,
Marcelo G. Dário
,
Eduarda H. Duval
,
Sergio L. Alves-Jr
,
Augusto Bücker
ORCID logo ,
Carlos A. Rosa
,
Barbara Dunn
,
Gavin Sherlock
* ORCID logo ,
Boris U. Stambuk
* ORCID logo
Version 1 : Received: 30 June 2023 / Approved: 5 July 2023 / Online: 6 July 2023 (02:17:42 CEST)

A peer-reviewed article of this Preprint also exists.

Muller, G.; de Godoy, V.R.; Dário, M.G.; Duval, E.H.; Alves-Jr, S.L.; Bücker, A.; Rosa, C.A.; Dunn, B.; Sherlock, G.; Stambuk, B.U. Improved Sugarcane-Based Fermentation Processes by an Industrial Fuel-Ethanol Yeast Strain. J. Fungi 2023, 9, 803. Muller, G.; de Godoy, V.R.; Dário, M.G.; Duval, E.H.; Alves-Jr, S.L.; Bücker, A.; Rosa, C.A.; Dunn, B.; Sherlock, G.; Stambuk, B.U. Improved Sugarcane-Based Fermentation Processes by an Industrial Fuel-Ethanol Yeast Strain. J. Fungi 2023, 9, 803.

Abstract

In Brazil, sucrose-rich broths (cane juice and/or molasses) are used to produce billions of liters of both fuel ethanol and cachaça per year using selected Saccharomyces cerevisiae industrial strains. Aiming to improve this disaccharide fermentation, we have first determined the genetic characteristics, by array comparative genomic hybridization, of a group of 8 fuel ethanol and 5 cachaça industrial yeasts that tend to dominate the fermentors during the production season. The widespread presence of SUC genes encoding invertase at multiple telomeres has been shown to be a common feature of both baker’s and distillers’ yeast strains and is postulated to be an adaptation to sucrose-rich broths. Our results show that only two strains (one fuel ethanol and one cachaça yeast) have amplification of the SUC genes, with high invertase activity. The other industrial yeast strains had a single SUC locus (SUC2) in their genome, with different patterns of invertase activity. These results indicate that invertase activity probably does not limit sucrose fermentation during fuel ethanol and cachaça production by these industrial strains. Using this knowledge, we changed the mode of sucrose metabolism of an industrial strain by avoiding extracellular invertase activity, overexpressing the intracellular invertase, and increasing its transport through the AGT1 permease. This approach allowed higher ethanol production from sucrose by the modified industrial yeast when compared to its parental strain.

Keywords

bioethanol; cachaça; sucrose; invertase; thiamin; Saccharomyces cerevisiae

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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