Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Biotechnological Potential of the Stress Response and Plant Cell Death Regulators Proteins in the Biofuel Industry

Maciej Jerzy Bernacki
,
Jakub Mielecki
ORCID logo ,
Andrzej Antczak
,
Michał Drożdżek
ORCID logo ,
Damin Witoń
,
Joanna Dąbrowska-Bronk
,
Piotr Gawroński
,
Paweł Burdiak
,
Monika Marchwicka
,
Anna Rusaczonek
ORCID logo ,
Katarzyna Dąbkowska-Susfał
,
Wacław Roman Strobel
,
Ewa Mellerowicz
,
Janusz Zawadzki
,
Magdalena Szechyńska-Hebda
,
Stanisław Karpiński
* ORCID logo
Version 1 : Received: 4 July 2023 / Approved: 5 July 2023 / Online: 6 July 2023 (03:28:59 CEST)

A peer-reviewed article of this Preprint also exists.

Bernacki, M.J.; Mielecki, J.; Antczak, A.; Drożdżek, M.; Witoń, D.; Dąbrowska-Bronk, J.; Gawroński, P.; Burdiak, P.; Marchwicka, M.; Rusaczonek, A.; Dąbkowska-Susfał, K.; Strobel, W.R.; Mellerowicz, E.J.; Zawadzki, J.; Szechyńska-Hebda, M.; Karpiński, S. Biotechnological Potential of the Stress Response and Plant Cell Death Regulators Proteins in the Biofuel Industry. Cells 2023, 12, 2018. Bernacki, M.J.; Mielecki, J.; Antczak, A.; Drożdżek, M.; Witoń, D.; Dąbrowska-Bronk, J.; Gawroński, P.; Burdiak, P.; Marchwicka, M.; Rusaczonek, A.; Dąbkowska-Susfał, K.; Strobel, W.R.; Mellerowicz, E.J.; Zawadzki, J.; Szechyńska-Hebda, M.; Karpiński, S. Biotechnological Potential of the Stress Response and Plant Cell Death Regulators Proteins in the Biofuel Industry. Cells 2023, 12, 2018.

Abstract

Production of biofuel from lignocellulosic biomass is relatively low due to the limited knowledge about natural cell wall loosening and cellulolytic processes in plants. Industrial separation of cellulose fiber mass from lignin, its saccharification and alcoholic fermentation is still cost-ineffective and environmental unfriendly. Assuming that the green transformation is inevitable and that the new sources of raw materials for biofuels are needed, we decided to study cell death - a natural process occurring in plants in a context of reducing the recalcitrance of lignocellulose for production of second generation bioethanol. “members of the enzyme families responsible for lysigenous aerenchyma formation were identified during the root hypoxia stress in Arabidopsis thaliana cell death mutants. The cell death regulatory genes, LESION SIMULATING DISEASE 1 (LSD1), PHYTOALEXIN DEFICIENT 4 (PAD4) and ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1) conditionally regulate the cell wall when suppressed in transgenic aspen. During four years of growth in the field the following effects were observed: lignin content was reduced, the cellulose fiber polymerization degree increased and the growth itself was unaffected. The wood of transgenic trees was more efficient as a substrate for saccharification, alcoholic fermentation and bioethanol production. The presented results may trigger the development of novel biotechnologies in the biofuel industry.

Keywords

stress response; poplar; lignin; bioethanol; cell wall; cell death; biofuels; stress response proteins

Subject

Biology and Life Sciences, Cell and Developmental Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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