Submitted:
01 July 2023
Posted:
04 July 2023
You are already at the latest version
Abstract
Keywords:
1. Introduction
2. Materials and Methods
2.1. Description of the study area
2.2. Experimental design and soil sampling
2.3. Statistical data analysis
3. Results
3.1. Effect of land use types and soil sampling depths on organic carbon and carbon management index
3.2. Effect of land use types on organic carbon and carbon management index at 0–15 cm soil depth
3.3. Effect of land use types on organic carbon and carbon management index at 15–30 cm soil depth
3.4. Regression analysis of the measured parameters
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
| S8 | S10 | S8 | S10 | S8 | S10 | ||||||||||
| 20 m | S7 | S7 | S7 | ||||||||||||
| S3 | S9 | S3 | S9 | S3 | S9 | ||||||||||
| S1 | S1 | S1 | |||||||||||||
| S6 | S5 | S6 | S5 | S6 | S5 | ||||||||||
| S2 | S4 | S2 | S4 | S2 | S4 | ||||||||||
| 50 m | 50 m | 50 m | |||||||||||||
| Replication 1 | Replication 2 | Replication 3 | |||||||||||||
Appendix B
| Land Use Type | Depth (cm) | Repl. | OC (%) | L-Treatment | L-Reference | Ref. Land Use | Carbon Pool Index (CPI) | LI | CMI | OM (%) |
|---|---|---|---|---|---|---|---|---|---|---|
| Tractor | 0-15 | 1 | 4.2 | 0.10 | 0.09 | 3.3 | 1.27 | 1.11 | 141.41 | 7.2 |
| Tractor | 15-30 | 1 | 3.1 | 0.08 | 0.07 | 2.7 | 1.15 | 1.14 | 131.22 | 5.3 |
| Tractor | 0-15 | 2 | 3.1 | 0.08 | 0.11 | 4.2 | 0.74 | 0.73 | 53.68 | 5.3 |
| Tractor | 15-30 | 2 | 2.5 | 0.06 | 0.06 | 2.3 | 1.09 | 1.00 | 108.70 | 4.3 |
| Tractor | 0-15 | 3 | 4.1 | 0.10 | 0.11 | 3.9 | 1.05 | 0.91 | 95.57 | 7.1 |
| Tractor | 15-30 | 3 | 3.8 | 0.09 | 0.08 | 2.8 | 1.36 | 1.13 | 152.68 | 6.6 |
| Tractor | 0-15 | 4 | 3.7 | 0.09 | 0.10 | 3.7 | 1.00 | 0.90 | 90.00 | 6.4 |
| Tractor | 15-30 | 4 | 3.6 | 0.09 | 0.07 | 2.6 | 1.38 | 1.29 | 178.02 | 6.2 |
| Tractor | 0-15 | 5 | 3.1 | 0.08 | 0.11 | 4 | 0.78 | 0.73 | 56.36 | 5.3 |
| Tractor | 15-30 | 5 | 2.7 | 0.07 | 0.06 | 2.3 | 1.17 | 1.17 | 136.96 | 4.7 |
| Tractor | 0-15 | 6 | 3.7 | 0.09 | 0.07 | 2.6 | 1.42 | 1.29 | 182.97 | 6.4 |
| Tractor | 15-30 | 6 | 3.1 | 0.08 | 0.06 | 2.2 | 1.41 | 1.33 | 187.88 | 5.3 |
| Hand hoe | 0-15 | 1 | 2.6 | 0.10 | 0.09 | 3.3 | 0.79 | 1.11 | 87.54 | 4.5 |
| Hand hoe | 15-30 | 1 | 1.9 | 0.09 | 0.07 | 2.7 | 0.70 | 1.29 | 90.48 | 3.3 |
| Hand hoe | 0-15 | 2 | 2.5 | 0.12 | 0.11 | 4.2 | 0.60 | 1.09 | 64.94 | 4.3 |
| Hand hoe | 15-30 | 2 | 1.8 | 0.10 | 0.06 | 2.3 | 0.78 | 1.67 | 130.43 | 3.1 |
| Hand hoe | 0-15 | 3 | 2.1 | 0.13 | 0.11 | 3.9 | 0.54 | 1.18 | 63.64 | 3.6 |
| Hand hoe | 15-30 | 3 | 1.5 | 0.11 | 0.08 | 2.8 | 0.54 | 1.38 | 73.66 | 2.6 |
| Hand hoe | 0-15 | 4 | 2 | 0.16 | 0.10 | 3.7 | 0.54 | 1.60 | 86.49 | 3.4 |
| Hand hoe | 15-30 | 4 | 1.9 | 0.18 | 0.07 | 2.6 | 0.73 | 2.57 | 187.91 | 3.3 |
| Hand hoe | 0-15 | 5 | 2.8 | 0.33 | 0.11 | 4 | 0.70 | 3.00 | 210.00 | 4.8 |
| Hand hoe | 15-30 | 5 | 1.7 | 0.29 | 0.06 | 2.3 | 0.74 | 4.83 | 357.25 | 2.9 |
| Hand hoe | 0-15 | 6 | 2.8 | 0.68 | 0.07 | 2.6 | 1.08 | 9.71 | 1046.15 | 4.8 |
| Hand hoe | 15-30 | 6 | 1.3 | 0.05 | 0.06 | 2.2 | 0.59 | 0.83 | 49.24 | 2.2 |
| Ranch | 0-15 | 1 | 2.6 | 0.10 | 0.09 | 3.3 | 0.79 | 1.11 | 87.54 | 4.5 |
| Ranch | 15-30 | 1 | 2.1 | 0.08 | 0.07 | 2.7 | 0.78 | 1.14 | 88.89 | 3.6 |
| Ranch | 0-15 | 2 | 3.1 | 0.11 | 0.11 | 4.2 | 0.74 | 1.00 | 73.81 | 5.3 |
| Ranch | 15-30 | 2 | 1.3 | 0.05 | 0.06 | 2.3 | 0.57 | 0.83 | 47.10 | 2.2 |
| Ranch | 0-15 | 3 | 2.7 | 0.10 | 0.11 | 3.9 | 0.69 | 0.91 | 62.94 | 4.7 |
| Ranch | 15-30 | 3 | 1.8 | 0.07 | 0.08 | 2.8 | 0.64 | 0.88 | 56.25 | 3.1 |
| Ranch | 0-15 | 4 | 3.6 | 0.13 | 0.10 | 3.7 | 0.97 | 1.30 | 126.49 | 6.2 |
| Ranch | 15-30 | 4 | 1.4 | 0.05 | 0.07 | 2.6 | 0.54 | 0.71 | 38.46 | 2.4 |
| Ranch | 0-15 | 5 | 2.9 | 0.11 | 0.11 | 4 | 0.73 | 1.00 | 72.50 | 5 |
| Ranch | 15-30 | 5 | 1.3 | 0.05 | 0.06 | 2.3 | 0.57 | 0.83 | 47.10 | 2.2 |
| Ranch | 0-15 | 6 | 2.5 | 0.09 | 0.07 | 2.6 | 0.96 | 1.29 | 123.63 | 4.3 |
| Ranch | 15-30 | 6 | 1.9 | 0.07 | 0.06 | 2.2 | 0.86 | 1.17 | 100.76 | 3.3 |
| Bare (Reference) | 0-15 | 1 | 3.3 | 0.09 | 0.09 | 3.3 | 1.00 | 1.00 | 100.00 | 5.7 |
| Bare (Reference) | 15-30 | 1 | 2.7 | 0.07 | 0.07 | 2.7 | 1.00 | 1.00 | 100.00 | 4.7 |
| Bare (Reference) | 0-15 | 2 | 4.2 | 0.11 | 0.11 | 4.2 | 1.00 | 1.00 | 100.00 | 7.2 |
| Bare (Reference) | 15-30 | 2 | 2.3 | 0.06 | 0.06 | 2.3 | 1.00 | 1.00 | 100.00 | 4 |
| Bare (Reference) | 0-15 | 3 | 3.9 | 0.11 | 0.11 | 3.9 | 1.00 | 1.00 | 100.00 | 6.7 |
| Bare (Reference) | 15-30 | 3 | 2.8 | 0.08 | 0.08 | 2.8 | 1.00 | 1.00 | 100.00 | 4.8 |
| Bare (Reference) | 0-15 | 4 | 3.7 | 0.10 | 0.10 | 3.7 | 1.00 | 1.00 | 100.00 | 6.4 |
| Bare (Reference) | 15-30 | 4 | 2.6 | 0.07 | 0.07 | 2.6 | 1.00 | 1.00 | 100.00 | 4.5 |
| Bare (Reference) | 0-15 | 5 | 4 | 0.11 | 0.11 | 4 | 1.00 | 1.00 | 100.00 | 6.9 |
| Bare (Reference) | 15-30 | 5 | 2.3 | 0.06 | 0.06 | 2.3 | 1.00 | 1.00 | 100.00 | 4 |
| Bare (Reference) | 0-15 | 6 | 2.6 | 0.07 | 0.07 | 2.6 | 1.00 | 1.00 | 100.00 | 4.5 |
| Bare (Reference) | 15-30 | 6 | 2.2 | 0.06 | 0.06 | 2.2 | 1.00 | 1.00 | 100.00 | 3.8 |
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| Measured Variables in Soils and Statistical Parameters | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Soil Organic Carbon | Soil Organic Matter | Carbon Management Index | ||||||||
| Source of Variation | d.f. | m.s. | v.r. | F pr. | m.s. | v.r. | F pr. | m.s. | v.r. | F pr. |
| Replication | 5 | 0.16 | 0.81 | 0.5 | 0.86 | 25681 | 1.65 | |||
| Land use types (T) | 3 | 4.72 | 24.03 | <0.001 | 14.15 | 24.23 | <0.001 | 36571 | 2.36 | 0.113 |
| Residual | 15 | 0.2 | 1.33 | 0.58 | 1.33 | 15522 | 0.64 | |||
| Depth (D) | 1 | 10.27 | 69.81 | <0.001 | 30.24 | 68.99 | <0.001 | 6596 | 0.27 | 0.607 |
| T×D | 3 | 0.35 | 2.36 | 0.102 | 1.06 | 2.41 | 0.097 | 13158 | 0.54 | 0.658 |
| Residual | 20 | 0.15 | 0.44 | 24210 | ||||||
| Total | 47 | |||||||||
| Factors Evaluated | Levels of Factors | Measured Parameters | ||
|---|---|---|---|---|
| SOC (%) | SOM (%) | CMI | ||
| Land use types | Tractor cultivated | 3.4a | 5.8a | 126.3a |
| Ranch land | 2.3b | 3.9b | 77.1a | |
| Hand hoe cultivated | 2.1b | 3.6b | 204a | |
| Bare land (Reference) | 3.1a | 5.3a | 100a | |
| P – value | <0.001 | <0.001 | 0.113 | |
| LSD (0.05) | 0.4 | 0.7 | 108.4 | |
| cv (%) | 11.6 | 11.6 | 26.7 | |
| Sampling depth (cm) | 0–15 | 3.2a | 5.4a | 139a |
| 15–30 | 2.2b | 3.9b | 115a | |
| P – value | <0.001 | <0.001 | 0.607 | |
| LSD (0.05) | 0.2 | 0.4 | 93.7 | |
| cv (%) | 2.6 | 2.7 | 12.4 | |
| Interactions | Tractor cultivated land (0–15 cm) | 3.7a | 6.3a | 103.3a |
| Tractor cultivated land (15–30 cm) | 3.1ab | 5.4ab | 149.2a | |
| Hand hoe (0–15 cm) | 2.5b | 4.2b | 259.8a | |
| Hand hoe cultivated (15–30 cm) | 1.7c | 2.9c | 148.2a | |
| Ranch land (0–15 cm) | 2.9ab | 5.0ab | 91.2a | |
| Ranch land (15–30 cm) | 1.6c | 2.8c | 63.1a | |
| Bare land (Reference) (0–15 cm) | 3.6a | 6.2a | 100a | |
| Bare land (Reference) (15–30 cm) | 2.5b | 4.3b | 100a | |
| P – value | 0.102 | 0.097 | 0.658 | |
| S.E.D. | 0.2 | 0.4 | 82.6 | |
| cv (%) | 15.2 | 14.3 | 21.3 | |
| Source | Measured Variables in Soils and Statistical Parameters | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| of | Soil Organic Carbon | Soil Organic Matter | Carbon Management Index | |||||||
| Variation | d.f. | m.s. | v.r. | F pr. | m.s. | v.r. | F pr. | m.s. | v.r. | F pr. |
| Replication | 5 | 0.067 | 0.26 | 0.1908 | 0.25 | 2405.6 | 2.95 | |||
| Land use types | 3 | 1.994 | 7.66 | 0.002 | 5.9804 | 7.73 | 0.002 | 4911.9 | 6.03 | 0.007 |
| Residual | 15 | 0.26 | 0.7734 | 814.7 | ||||||
| Total | 23 | |||||||||
| Source | Measured Variables in Soils and Statistical Parameters | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| of | Soil Organic Carbon | Soil Organic Matter | Carbon Management Index | |||||||
| Variation | d.f. | m.s. | v.r. | F pr. | m.s. | v.r. | F pr. | m.s. | v.r. | F pr. |
| Replication | 5 | 0.21 | 2.2 | 0.63 | 2.18 | 240.1 | 0.52 | |||
| Land use types | 3 | 3.07 | 32.66 | <0.001 | 9.22 | 31.9 | <0.001 | 18127 | 39 | <0.001 |
| Residual | 15 | 0.09 | 0.29 | 464.8 | ||||||
| Total | 23 | |||||||||
| Land Use Types | Measured Parameters in Soils | ||
|---|---|---|---|
| Soil organic carbon | Soil Organic Matter | Carbon Management Index | |
| Tractor | 3.1a | 5.4a | 161.8a |
| Ranch | 1.6c | 2.8c | 45.1c |
| Hand hoe | 1.7c | 2.9c | 47.8c |
| Bare (Reference) | 2.5b | 4.3b | 101.1b |
| P - value | <0.001 | <0.001 | <0.001 |
| LSD(0.05) | 0.4 | 0.7 | 26.5 |
| cv (%) | 13.7 | 14 | 24.2 |
| Land Use Types | |||
|---|---|---|---|
| Regression Statistics | Tractor | Hand Hoe | Ranch |
| Multiple R | 0.997 | 0.991 | 0.998 |
| R Square | 0.993 | 0.983 | 0.996 |
| Adjusted R Square | 0.989 | 0.973 | 0.994 |
| Standard Error | 4.828 | 46.096 | 2.177 |
| Observations | 12 | 12 | 12 |
| Tractor | Hand Hoe | Ranch | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| df | MS | F-stat. | Significance F | MS | F-stat. | Significance F | MS | F-stat. | Significance F | |
| Regression | 4 | 5828.44 | 250.03 | 1.25E-07 | 210945 | 99.28 | 3.04E-06 | 2334.57 | 492.43 | 1.19E-08 |
| Residual | 7 | 23.31 | 2124.84 | 4.74 | ||||||
| Total | 11 | |||||||||
| Land Use Type | Fitted Parameters | Coefficients | Standard Error | t Stat | p-Value | Lower 95% | Upper 95% |
|---|---|---|---|---|---|---|---|
| Tractor | Intercept | -109.67 | 14.52 | -7.55 | 0.00013 | -144.01 | -75.33 |
| SOC | 84.49 | 75.4 | 1.12 | 0.29945 | -93.8 | 262.77 | |
| CPI | 100.52 | 34.66 | 2.9 | 0.02298 | 18.56 | 182.47 | |
| LI | 112.67 | 36.87 | 3.06 | 0.01842 | 25.5 | 199.85 | |
| SOM | -48.91 | 44.04 | -1.11 | 0.30345 | -153.06 | 55.23 | |
| Hand hoe | Intercept | -214.91 | 113.41 | -1.9 | 0.09993 | -483.1 | 53.3 |
| SOC | 147.65 | 1163.96 | 0.13 | 0.90263 | -2604.7 | 2900 | |
| CPI | 223.28 | 196.7 | 1.14 | 0.2937 | -241.8 | 688.4 | |
| LI | 97.17 | 11.81 | 8.23 | 7.63E-05 | 69.2 | 125.1 | |
| SOM | -80.57 | 676.47 | -0.12 | 0.9085369 | -1680.2 | 1519 | |
| Ranch | Intercept | -74.88 | 3.76 | -19.93 | 0.0000002 | -83.8 | -66 |
| SOC | 82.62 | 48.04 | 1.72 | 0.12914 | -31 | 196.2 | |
| CPI | 170.5 | 28.58 | 5.97 | 0.00056 | 102.9 | 238.1 | |
| LI | 30.95 | 20.38 | 1.52 | 0.1725 | -17.2 | 79.1 | |
| SOM | -49.26 | 27.89 | -1.77 | 0.12072 | -115.2 | 16.7 |
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