Version 1
: Received: 27 June 2023 / Approved: 28 June 2023 / Online: 28 June 2023 (07:23:02 CEST)
How to cite:
Ghiaee Shamloo, A.; Yadegari, M. H.; Jaafari, M. R.; Zarrinfar, H. Inhibitory Effect Evaluation of Nigella sativa Oil Loaded to Liposomal Nanocarriers against Candida parapsilosis Isolates. Preprints2023, 2023061953. https://doi.org/10.20944/preprints202306.1953.v1
Ghiaee Shamloo, A.; Yadegari, M. H.; Jaafari, M. R.; Zarrinfar, H. Inhibitory Effect Evaluation of Nigella sativa Oil Loaded to Liposomal Nanocarriers against Candida parapsilosis Isolates. Preprints 2023, 2023061953. https://doi.org/10.20944/preprints202306.1953.v1
Ghiaee Shamloo, A.; Yadegari, M. H.; Jaafari, M. R.; Zarrinfar, H. Inhibitory Effect Evaluation of Nigella sativa Oil Loaded to Liposomal Nanocarriers against Candida parapsilosis Isolates. Preprints2023, 2023061953. https://doi.org/10.20944/preprints202306.1953.v1
APA Style
Ghiaee Shamloo, A., Yadegari, M. H., Jaafari, M. R., & Zarrinfar, H. (2023). Inhibitory Effect Evaluation of <em>Nigella sativa</em> Oil Loaded to Liposomal Nanocarriers against<em> Candida parapsilosis</em> Isolates. Preprints. https://doi.org/10.20944/preprints202306.1953.v1
Chicago/Turabian Style
Ghiaee Shamloo, A., Mahmoud Reza Jaafari and Hossein Zarrinfar. 2023 "Inhibitory Effect Evaluation of <em>Nigella sativa</em> Oil Loaded to Liposomal Nanocarriers against<em> Candida parapsilosis</em> Isolates" Preprints. https://doi.org/10.20944/preprints202306.1953.v1
Abstract
The aim of current study is adjusted and synthesized liposomal compound of N. sativa and evaluation its antifungal properties against C. parapsilosis isolates. Fifteen clinical isolates of C. parapsilosis complex isolates were obtained from hospitalized patients affected by candidemia in Mashhad city, Iran, along with a reference strain of C. parapsilosis (ATCC 22019) were assessed by flight mass spectrometry (MALDI-TOF) method, as described previously. N. sativa is encapsulated in liposomal Nanocariers by using thin film hydration technique. At the beginning liposomal nanoparticles was characterized and confirmed with the dynamic light scattering technique (DLS) and Transmission electron microscopy. Then minimum inhibitory concentration of liposomal N. sativa oil was conducted with the CLSI M27 A3 protocol and finally Cytotoxicity function of N. sativa oil liposomal nanocarriers on PBMCs was investigated and confirmed with MTT assay by the results of this research N. sativa oil-Lip-NP didn’t show any toxic effect on PBMCs and The minimum inhibitory concentration (MIC) range of free N. sativa oil and liposomal formulation with inhibitory effects on candida isolates was between 128 - 8, 250 - 31.25 µg ml also MIC50 and MIC90 were 125,187 and 32,96, µg ml respectively. Due to the hydrophobicity and hydrophilicity, biocompatibility, particle size, non-toxic effect, and higher cell viability of N. sativa oil -Lip-NP, it could be considered a more effective approach to treating fungal infections.
Medicine and Pharmacology, Medicine and Pharmacology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
