Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Proteome and Interactome Linked to Metabolism, Genetic Information Processing, and Abiotic Stress in Gametophytes of Two Woodferns

Sara Ojosnegros
,
José Manuel Alvarez
ORCID logo ,
Jonas Grossmann
,
Valeria Gagliardini
,
Luis G. Quintanilla
ORCID logo ,
Ueli Grossniklaus
ORCID logo ,
Helena Fernández
* ORCID logo
Version 1 : Received: 26 June 2023 / Approved: 27 June 2023 / Online: 27 June 2023 (04:44:31 CEST)

A peer-reviewed article of this Preprint also exists.

Ojosnegros, S.; Alvarez, J.M.; Grossmann, J.; Gagliardini, V.; Quintanilla, L.G.; Grossniklaus, U.; Fernández, H. Proteome and Interactome Linked to Metabolism, Genetic Information Processing, and Abiotic Stress in Gametophytes of Two Woodferns. Int. J. Mol. Sci. 2023, 24, 12429. Ojosnegros, S.; Alvarez, J.M.; Grossmann, J.; Gagliardini, V.; Quintanilla, L.G.; Grossniklaus, U.; Fernández, H. Proteome and Interactome Linked to Metabolism, Genetic Information Processing, and Abiotic Stress in Gametophytes of Two Woodferns. Int. J. Mol. Sci. 2023, 24, 12429.

Abstract

Ferns and lycophytes, now known as monilophytes, have received scant molecular attention in comparison to angiosperms. The advent of high-throughput technologies allowed an advance towards a greater knowledge of their elusive genomes. In this work, proteomic analyses of heart-shaped gametophytes of two ferns were performed: the apomictic species Dryopteris affinis ssp. affinis and its sexual relative Dryopteris oreades. In total, a set of 218 proteins shared by these two gametophytes were analyzed using the STRING database, and the proteome associated with metabolism, genetic information processing, and responses to abiotic stress is discussed. Specifi-cally, we report proteins involved in the metabolism of carbohydrates, lipids, and nucleotides, the biosynthesis of amino acids, and secondary compounds, energy, oxido-reduction, transcrip-tion, translation, protein folding, sorting and degradation, and responses to abiotic stress. Look-ing at the interactome of this set of proteins, it represents a total network composed of 218 nodes and 1,792 interactions, obtained mostly from databases and textmining. The interactions among the identified proteins of the ferns D. affinis and D. oreades, together with the description of their biological functions, might contribute to a better understanding of the function and develop-ment of ferns as well as to fill knowledge gaps in plant evolution.

Keywords

Dryopteris affinis ssp. affinis; Dryopteris oreades; fern; gametophyte; non-seed plants; proteome; STRING database.

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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