Version 1
: Received: 23 June 2023 / Approved: 26 June 2023 / Online: 26 June 2023 (11:14:25 CEST)
How to cite:
Aline, S.; Koller, M.; Baumert, R.; Hautz, J.; Treiber, F.M.; Kittinger, C.; Zarfel, G. Comparison of Antibiotic Resistance of Escherichia coli Populations from Water or Sediment in Rivers Environments. Preprints2023, 2023061800. https://doi.org/10.20944/preprints202306.1800.v1
Aline, S.; Koller, M.; Baumert, R.; Hautz, J.; Treiber, F.M.; Kittinger, C.; Zarfel, G. Comparison of Antibiotic Resistance of Escherichia coli Populations from Water or Sediment in Rivers Environments. Preprints 2023, 2023061800. https://doi.org/10.20944/preprints202306.1800.v1
Aline, S.; Koller, M.; Baumert, R.; Hautz, J.; Treiber, F.M.; Kittinger, C.; Zarfel, G. Comparison of Antibiotic Resistance of Escherichia coli Populations from Water or Sediment in Rivers Environments. Preprints2023, 2023061800. https://doi.org/10.20944/preprints202306.1800.v1
APA Style
Aline, S., Koller, M., Baumert, R., Hautz, J., Treiber, F.M., Kittinger, C., & Zarfel, G. (2023). Comparison of Antibiotic Resistance of <em>Escherichia coli</em> Populations from Water or Sediment in Rivers Environments. Preprints. https://doi.org/10.20944/preprints202306.1800.v1
Chicago/Turabian Style
Aline, S., Clemens Kittinger and Gernot Zarfel. 2023 "Comparison of Antibiotic Resistance of <em>Escherichia coli</em> Populations from Water or Sediment in Rivers Environments" Preprints. https://doi.org/10.20944/preprints202306.1800.v1
Abstract
The detection of antibiotic-resistant facultative pathogenic bacteria in surface waters is common in the present day. However, there is limited understanding of the factors that influence the spread and stabilization of resistance in this habitat, particularly regarding the role of biofilms. Despite the perceived differences between sediments, biofilms, and open water, their potential contribution to the long-term maintenance of resistances remains unclear. In this study, we investigated Escherichia coli isolates obtained from the Mur and Drava rivers in Austria. Samples were collected from both the water column and sediment at two locations per river: upstream and downstream of an urban area, including a sewage treatment plant. The iso-lates were subjected to antimicrobial susceptibility testing against 21 antibiotics belonging to seven distinct classes. Additionally, any isolates exhibiting an extended-spectrum beta-lactamase (ESBL) or carbapenemase phenotype were further analyzed for the presence of specific antimi-crobial resistance genes. E. coli isolates obtained from the two rivers exhibited resistance to at least one of the tested anti-biotics, with rates of 25.83% and 23.66% respectively. The most prevalent antimicrobial re-sistances observed were towards ampicillin, amoxicillin-clavulanic acid, tetracycline, and na-lidixic acid. Surprisingly, there was a similar proportion of resistant bacteria observed in both open water and sediment samples, contrary to expectations. The difference in resistance levels between samples collected upstream and downstream of the cities was minimal. Out of the total isolates, 13 E. coli were found to carry ESBL genes with one isolate carrying the gene for KPC-2 carbapenemase. There were no significant differences between the biofilm (sediment) and open water samples in the occurrence of antimicrobial resistance. Even the difference between the samples upstream and downstream of the cities and their wastewater treatment plants was minor. There seems to be no evidence of a large adaptive effect in this analysed biofilm population of E. coli. The un-typical phenotype of the KPC-producing and some of the ESBL-producing E. coli from this study could, however, be an indication that such adaptation effects generally exist in water.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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