Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Polydopamine-Coated Polycaprolactone Electrospun Nanofiber Membrane Loaded with Thrombin for Wound Hemostasis

Dapeng Cui
† ,
Ming Li
† ORCID logo ,
Peng Zhang
,
Feng Rao
,
Wei Huang
,
Chuanlin Wang
,
Wei Guo
* ,
Tianbing Wang
*
†
Dapeng Cui and Ming Li contributed equally to this work.
Version 1 : Received: 20 June 2023 / Approved: 25 June 2023 / Online: 25 June 2023 (02:08:31 CEST)

A peer-reviewed article of this Preprint also exists.

Cui, D.; Li, M.; Zhang, P.; Rao, F.; Huang, W.; Wang, C.; Guo, W.; Wang, T. Polydopamine-Coated Polycaprolactone Electrospun Nanofiber Membrane Loaded with Thrombin for Wound Hemostasis. Polymers 2023, 15, 3122. Cui, D.; Li, M.; Zhang, P.; Rao, F.; Huang, W.; Wang, C.; Guo, W.; Wang, T. Polydopamine-Coated Polycaprolactone Electrospun Nanofiber Membrane Loaded with Thrombin for Wound Hemostasis. Polymers 2023, 15, 3122.

Abstract

Hemorrhagic shock is the primary cause of death in patients with severe trauma, and the development of rapid and efficient hemostatic methods is of great significance in promoting the life-saving of trauma patients. In this study, polycaprolactone (PCL) nanofiber membrane was prepared by electrospinning technology. The PCL-PDA loading system was developed by modifying the surface of polydopamine (PDA) inspired by mussel adhesion protein, and the efficient and stable loading of thrombin (TB) was realized on the premise of ensuring the bioactivity of TB. The new thrombin loading system overcomes the disadvantages of harsh storage conditions, poor strength, easy to fall off, and can use thrombin to start rapid coagulation cascade reaction, which has the characteristics of fast hemostasis, good biocompatibility, high safety and wide range of hemostasis. The physicochemical properties and biocompatibility of PCL-PDA-TB membrane were verified by scanning electron microscopy, cell proliferation test, cell adhesion test and extract cytotoxicity test. Red blood cell adhesion, platelet adhesion, dynamic coagulation time and animal models all verified the coagulation effect of PCL-PD-TB membrane. Therefore, PCL-PDA-TB membrane has great potential in wound hemostasis.

Keywords

Trauma; Electrospinning technology; Polydopamine modification; Thrombin; Hemostatic method

Subject

Chemistry and Materials Science, Biomaterials

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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