PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Exploring the Evolutionary Relics of Sex-chromosomes of Divergent Animal Species through Comparative Analysis of the Predicted SSR Sequences and CpG Islands
Version 1
: Received: 15 June 2023 / Approved: 16 June 2023 / Online: 16 June 2023 (12:41:59 CEST)
How to cite:
Grewal, B.S.; Tewari, S.; Hægland, H.; Mukhopadhyay, C.S. Exploring the Evolutionary Relics of Sex-chromosomes of Divergent Animal Species through Comparative Analysis of the Predicted SSR Sequences and CpG Islands. Preprints2023, 2023061242. https://doi.org/10.20944/preprints202306.1242.v1
Grewal, B.S.; Tewari, S.; Hægland, H.; Mukhopadhyay, C.S. Exploring the Evolutionary Relics of Sex-chromosomes of Divergent Animal Species through Comparative Analysis of the Predicted SSR Sequences and CpG Islands. Preprints 2023, 2023061242. https://doi.org/10.20944/preprints202306.1242.v1
Grewal, B.S.; Tewari, S.; Hægland, H.; Mukhopadhyay, C.S. Exploring the Evolutionary Relics of Sex-chromosomes of Divergent Animal Species through Comparative Analysis of the Predicted SSR Sequences and CpG Islands. Preprints2023, 2023061242. https://doi.org/10.20944/preprints202306.1242.v1
APA Style
Grewal, B.S., Tewari, S., Hægland, H., & Mukhopadhyay, C.S. (2023). Exploring the Evolutionary Relics of Sex-chromosomes of Divergent Animal Species through Comparative Analysis of the Predicted SSR Sequences and CpG Islands. Preprints. https://doi.org/10.20944/preprints202306.1242.v1
Chicago/Turabian Style
Grewal, B.S., Håkon Hægland and Chandra Shekhar Mukhopadhyay. 2023 "Exploring the Evolutionary Relics of Sex-chromosomes of Divergent Animal Species through Comparative Analysis of the Predicted SSR Sequences and CpG Islands" Preprints. https://doi.org/10.20944/preprints202306.1242.v1
Abstract
DNA markers have high occurrence and mutation rates and are generally located around the controlling regions of some tissue-specific genes and housekeeping genes that can change the expression pattern. Microsatellites and CpG islands are stretches of DNA with repeats and are known to influence gene expression. Microsatellites are more prone to mutations than the rest of the genomic DNA which allows the straightforward genomic nucleotide evolutionary transformation rate in different species. In the present study, these DNA markers are mined and an in-silico comparison was carried out to understand their occurrence pattern and distribution frequency in sex chromosomes (X and Y) of 12 different animal species using Perl and R programming pipelines. It was found that female-dominant X chromosomes had higher occurrence and distribution frequencies for these DNA markers than that of male-dominant sex chromosome i.e. Y which means that the former has a higher number of the evolutionary sites. The density of DNA markers however, showed remarkable variation for different animal species The results obtained need validation through wet-lab experimentation. Tri- and hexa-nucleotide repeats are more abundant in exons, whereas other repeats are more abundant in non-coding regions.
Keywords
Microsatellite; CpG island; sex chromosomes; DNA markers
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
