Moghaddam, E.; Zandi, K.; Lani, R.; Erwandi, D.; Chik, Z.; Bakar, S.A.; Hassandarvish, P. Sera of Rats Fed With Baicalein Showed Effective Against Dengue Virus Replication. Preprints2023, 2023060906. https://doi.org/10.20944/preprints202306.0906.v1
APA Style
Moghaddam, E., Zandi, K., Lani, R., Erwandi, D., Chik, Z., Bakar, S.A., & Hassandarvish, P. (2023). Sera of Rats Fed With Baicalein Showed Effective Against Dengue Virus Replication. Preprints. https://doi.org/10.20944/preprints202306.0906.v1
Chicago/Turabian Style
Moghaddam, E., Sazaly Abu Bakar and Pouya Hassandarvish. 2023 "Sera of Rats Fed With Baicalein Showed Effective Against Dengue Virus Replication" Preprints. https://doi.org/10.20944/preprints202306.0906.v1
Abstract
Dengue virus (DENV) is a member of the Flaviviridae family and is responsible for the most common mosquito-borne human disease. Currently, there are no available antiviral drugs to treat DENV infections. However, certain flavonoids, including baicalein and baicalin, have demon-strated significant anti-DENV effects in vitro. This study aimed to assess the bioavailability of baicalein and its metabolite, baicalin, in the blood serum of Albino Wistar rats using the LC/MS/MS method. The in vitro activity of sera obtained from rats administered baicalein was evaluated for its anti-DENV properties using quantitative RT-PCR and the Foci Forming Reduction Assay (FFRA). The results obtained through LC/MS/MS analysis indicated that the bioavailability of baicalin in rat blood serum reached 44.20 µg/ml one hour after oral administration of baicalein. Furthermore, the sera collected from rats given baicalein exhibited a significant 78.26% inhibition of DENV-2 replication when tested against Dengue-2 using both quantitative RT-PCR and FFRA. These findings suggest that baicalein and baicalin hold promise as potential therapeutic candidates for further investigation in the development of treatments against DENV infections.
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