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A Comparative Study on Kinetics Performances of BSA-gold Nanozymes for Nanozyme-Mediated Oxidation of 3,3’,5,5’-Tetramethylbenzidine and 3,3’-Diaminobenzidine
Hormozi Jangi SR (2023) A Comparative Study on Kinetics Performances of BSA-gold Nanozymes for Nanozyme-mediated Oxidation of 3,3',5,5'-Tetramethylbenzidine and 3,3'-Diaminobenzidine. Biochem Mol Biol J. 9:21.
Hormozi Jangi SR (2023) A Comparative Study on Kinetics Performances of BSA-gold Nanozymes for Nanozyme-mediated Oxidation of 3,3',5,5'-Tetramethylbenzidine and 3,3'-Diaminobenzidine. Biochem Mol Biol J. 9:21.
Hormozi Jangi SR (2023) A Comparative Study on Kinetics Performances of BSA-gold Nanozymes for Nanozyme-mediated Oxidation of 3,3',5,5'-Tetramethylbenzidine and 3,3'-Diaminobenzidine. Biochem Mol Biol J. 9:21.
Hormozi Jangi SR (2023) A Comparative Study on Kinetics Performances of BSA-gold Nanozymes for Nanozyme-mediated Oxidation of 3,3',5,5'-Tetramethylbenzidine and 3,3'-Diaminobenzidine. Biochem Mol Biol J. 9:21.
Abstract
It is well-known that the peroxidase enzyme catalyzes the oxidation of different substrates such as 3,3’,5,5’-thetramethylbenzidine, and 3,3’-diaminobenzidine, however, the affinity of different substrates to the enzyme and consequently and catalytic performances of the enzyme toward different substrates is not same, resulting in different kinetics indexes. As the BSA-gold nanozymes are considered artificial peroxidase enzymes, hence, the kinetics indexes of these nanozymes should also be varied by varying their substrate. Hence, in this work, a comparative study was performed on the kinetics performances of BSA-gold nanozymes for enzyme-mediated oxidations of 3,3’,5,5’-thetramethylbenzidine, and 3,3’-diaminobenzidine. The results showed that the Km value of BSA-gold nanozymes was 0.03 mM and 0.72 mM toward TMB and DAB, in order, to reveal the higher affinity (lower Km) of TMB for binding to nanozyme active nodes compared to its alternative substrate, DAB. In contrast, the Vmax was found to be 263 nM sec-1 and 185 nM sec-1 for nanozmye-mediated oxidation of TMB and DAB, respectively. The higher Vmax of the nanozyme-mediated oxidation of TMB revealed that the catalytic efficiency of BSA-Au nanozymes toward TMB oxidation is higher (about 1.5-fold) than that of the DAB oxidation. The difference between the kinetic indexes of TMB and DAB may be related to their different oxidation pathways and their different reactivity. In fact, the DAB oxidizes via an n-electron irreversible oxidation pathway to produce an indamine polymer. While TMB nanozyme-mediated oxidation has occurred upon a 2-electron reversible mechanism for the production of a cation radical. These different pathways resulted in different kinetic performances.
Chemistry and Materials Science, Analytical Chemistry
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