Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Sulforaphane Reduces Chronic Inflammatory Immune Response of Human Dendritic Cells

Laura Fernández-Prades
,
Mariano Brasal-Prieto
,
Gonzalo Alba
ORCID logo ,
Victoria Martín
,
Sergio Montserrat-de la Paz
ORCID logo ,
Marta Cejudo-Guillen
,
Consuelo Santa-Maria
ORCID logo ,
Hala Dakhaoui
ORCID logo ,
Beatriz Granados
,
Francisco Sobrino
,
Francisca Palomares
* ,
Soledad Lopez-Enriquez
* ORCID logo
Version 1 : Received: 5 June 2023 / Approved: 5 June 2023 / Online: 5 June 2023 (12:50:29 CEST)

A peer-reviewed article of this Preprint also exists.

Fernandez-Prades, L.; Brasal-Prieto, M.; Alba, G.; Martin, V.; Montserrat-de la Paz, S.; Cejudo-Guillen, M.; Santa-Maria, C.; Dakhaoui, H.; Granados, B.; Sobrino, F.; Palomares, F.; Lopez-Enriquez, S. Sulforaphane Reduces the Chronic Inflammatory Immune Response of Human Dendritic Cells. Nutrients 2023, 15, 3405. Fernandez-Prades, L.; Brasal-Prieto, M.; Alba, G.; Martin, V.; Montserrat-de la Paz, S.; Cejudo-Guillen, M.; Santa-Maria, C.; Dakhaoui, H.; Granados, B.; Sobrino, F.; Palomares, F.; Lopez-Enriquez, S. Sulforaphane Reduces the Chronic Inflammatory Immune Response of Human Dendritic Cells. Nutrients 2023, 15, 3405.

Abstract

Background: Sulforaphane (SFN) is an isothiocyanate of vegetables origin with potent antioxidant and immunomodulatory properties. The pleiotropic activities characterization in human dendritic cells (DCs) is poorly summarized. The aim of this work was the study of the immunomodulatory power of SFN in response to an inflammatory microenvironment on human monocyte derived DCs (moDCs). Methods: The immunological response induced by SFN was studied, evaluating apoptosis and autophagy assays by flow cytometry in moDCs and cancer cell line (THP-1), including moDCs maturation, lymphocyte proliferation and cytokines production under different experimental conditions associated or not with an inflammatory microenvironment, which was induced by lipopolysaccharide (LPS). Results: Our results demonstrated that SFN can interact with moDCs, significantly reducing autophagy process and enhancing apoptosis, such as THP-1 cells, in chronic inflammatory microenvironment. Under this chronic inflammation, SFN modulated the phenotypical characteristics of moDCs, which reduction the expression of all markers (CD80, CD83, CD86, HLA-DR and PD-L1), and significantly reduced the Th2 proliferative response together with the reduction of the IL-9 and IL-13 levels. Although we did not find changes in the regulatory proliferative response, we observed an increase in IL-10 levels. Conclusion: These findings demonstrate that SFN exerts protective effects against LPS-induced inflammation through moDCs/T-cells modulation towards a regulatory profile. Therefore, SFN may be a potential candidate for use in the treatment of pathologies with an inflammatory profile.

Keywords

nutraceutic; dendritic cells; T lymphocytes; anti-inflammatory response; autophagy; apoptosis

Subject

Medicine and Pharmacology, Dietetics and Nutrition

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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