da Silva, V.L.; Mota, G.A.F.; de Souza, S.L.B.; de Campos, D.H.S.; Melo, A.B.; Vileigas, D.F.; Coelho, P.M.; Sant’Ana, P.G.; Padovani, C.; Lima-Leopoldo, A.P.; Bazan, S.G.Z.; Leopoldo, A.S.; Cicogna, A.C. Aerobic Exercise Training Improves Calcium Handling and Cardiac Function in Rats with Heart Failure Resulting from Aortic Stenosis. Int. J. Mol. Sci.2023, 24, 12306.
da Silva, V.L.; Mota, G.A.F.; de Souza, S.L.B.; de Campos, D.H.S.; Melo, A.B.; Vileigas, D.F.; Coelho, P.M.; Sant’Ana, P.G.; Padovani, C.; Lima-Leopoldo, A.P.; Bazan, S.G.Z.; Leopoldo, A.S.; Cicogna, A.C. Aerobic Exercise Training Improves Calcium Handling and Cardiac Function in Rats with Heart Failure Resulting from Aortic Stenosis. Int. J. Mol. Sci. 2023, 24, 12306.
da Silva, V.L.; Mota, G.A.F.; de Souza, S.L.B.; de Campos, D.H.S.; Melo, A.B.; Vileigas, D.F.; Coelho, P.M.; Sant’Ana, P.G.; Padovani, C.; Lima-Leopoldo, A.P.; Bazan, S.G.Z.; Leopoldo, A.S.; Cicogna, A.C. Aerobic Exercise Training Improves Calcium Handling and Cardiac Function in Rats with Heart Failure Resulting from Aortic Stenosis. Int. J. Mol. Sci.2023, 24, 12306.
da Silva, V.L.; Mota, G.A.F.; de Souza, S.L.B.; de Campos, D.H.S.; Melo, A.B.; Vileigas, D.F.; Coelho, P.M.; Sant’Ana, P.G.; Padovani, C.; Lima-Leopoldo, A.P.; Bazan, S.G.Z.; Leopoldo, A.S.; Cicogna, A.C. Aerobic Exercise Training Improves Calcium Handling and Cardiac Function in Rats with Heart Failure Resulting from Aortic Stenosis. Int. J. Mol. Sci. 2023, 24, 12306.
Abstract
Aerobic exercise training (AET) has been used in the management of heart disease. AET may, totally or partially, restore the activity and/or expression of proteins that regulate calcium (Ca2+) handling, optimize intracellular Ca2+ flow, and attenuate cardiac functional impairment in failing hearts. However, the literature presents conflicting data regarding the effects of AET on Ca2+ transit and cardiac function in rats with heart failure resulting from aortic stenosis. The objective of this study was to evaluate the effects of AET on calcium handling and cardiac function in rats with heart failure due to aortic stenosis. Wistar rats were distributed into two groups: control (Sham; n= 61) and aortic stenosis (AoS; n= 44). After 18 weeks, the groups were redistributed into: non-exposed to exercise training (Sham, n = 28 and AoS, n = 22) and trained (Sham-ET, n = 33 and AoS-ET, n = 22) for 10 weeks. Treadmill exercise training was performed with a velocity equivalent to the lactate threshold. Echocardiogram, isolated papillary muscle, and isolated cardiomyocyte analyzed cardiac function. During isolated papillary muscle assay and isolated cardimyocyte was evaluated Ca2+. The expression of regulatory proteins of diastolic Ca2+ was analyzed via Western Blot. AET attenuated the diastolic dysfunction and benefited the systolic function. AoS-ET animals presented better response to post-rest contraction, and SERCA2a and L-type Ca2+ channels blocked than the AoS. Furthermore, AET was able to improve aspects of the mechanical function and the responsiveness of the myofilaments to the Ca2+ of the AoS-ET animals. AoS animals presented alteration in the protein expression of the SERCA2a and NCX, and AET restored SERCA2a and NCX levels near normal values. Therefore, AET increased SERCA2a activity, improved the cellular Ca2+ influx mechanism, and increased myofilament responsiveness to Ca2+, attenuating cardiac dysfunction at cellular, tissue, and chamber levels in animals with aortic stenosis and heart failure.
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