Rtishchev, A.; Treshchalina, A.; Shustova, E.; Boravleva, E.; Gambaryan, A. An Outbreak of Newcastle Disease Virus in the Moscow Region in the Summer of 2022. Vet. Sci.2023, 10, 404.
Rtishchev, A.; Treshchalina, A.; Shustova, E.; Boravleva, E.; Gambaryan, A. An Outbreak of Newcastle Disease Virus in the Moscow Region in the Summer of 2022. Vet. Sci. 2023, 10, 404.
Rtishchev, A.; Treshchalina, A.; Shustova, E.; Boravleva, E.; Gambaryan, A. An Outbreak of Newcastle Disease Virus in the Moscow Region in the Summer of 2022. Vet. Sci.2023, 10, 404.
Rtishchev, A.; Treshchalina, A.; Shustova, E.; Boravleva, E.; Gambaryan, A. An Outbreak of Newcastle Disease Virus in the Moscow Region in the Summer of 2022. Vet. Sci. 2023, 10, 404.
Abstract
In August 2022, in the Moscow region, Russia, on a backyard farm, the mortality was observed at chickens and all 45 birds of this farm died or were slaughtered after onset of symptoms within a few days. Paramyxovirus was isolated from diseased birds. Based on the nucleotide sequences of the F and NP gene fragments, the belonging of virus to subgenotype VII.1 AAvV-1 class II was established. The cleavage site of the F gene 109SGGRRQKRFIG119 and T in 546 and 555 position of NP gene, were typical for the velogenic type. The genetically closest NDV isolates were found in Iran. The mean time of death of 10-day-old chicken embryos upon infection with the minimal infectious dose was 52 hours, which is typical for the velogenic pathotype. The virus caused 100% death of six-week-old chickens during oral infection, as well as 100% mortality of all contact chickens, including those located in remote cages, which proves the ability of virus to spread not only by fecal-oral but also by aerosol route. That demonstrates a high level of pathogenicity and contagiousness of the isolated strain for chicken. However, the mice intranasally infected with high doses of the virus had not die.
Keywords
NDV; paramyxovirus; outbreak; pathogenicity
Subject
Biology and Life Sciences, Virology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
