Hadzega, D.; Babisova, K.; Hyblova, M.; Janostiakova, N.; Sabaka, P.; Janega, P.; Minarik, G. Analysis of Transcriptomics Data from COVID-19 Patients: A Pilot Research. Folia Microbiologica 2024, doi:10.1007/s12223-024-01130-x.
Hadzega, D.; Babisova, K.; Hyblova, M.; Janostiakova, N.; Sabaka, P.; Janega, P.; Minarik, G. Analysis of Transcriptomics Data from COVID-19 Patients: A Pilot Research. Folia Microbiologica 2024, doi:10.1007/s12223-024-01130-x.
Hadzega, D.; Babisova, K.; Hyblova, M.; Janostiakova, N.; Sabaka, P.; Janega, P.; Minarik, G. Analysis of Transcriptomics Data from COVID-19 Patients: A Pilot Research. Folia Microbiologica 2024, doi:10.1007/s12223-024-01130-x.
Hadzega, D.; Babisova, K.; Hyblova, M.; Janostiakova, N.; Sabaka, P.; Janega, P.; Minarik, G. Analysis of Transcriptomics Data from COVID-19 Patients: A Pilot Research. Folia Microbiologica 2024, doi:10.1007/s12223-024-01130-x.
Abstract
During the infection by SARS-CoV-2, the virus is changing infected host cell into its own factory producing new viral particles. As infection progresses, infected cell undergoes many changes in various pathways. One of the events caused by changes is cytokine storm, which leads to the severe symptoms. In this study, we investigated transcriptomic changes caused by COVID-19 disease using RNA-seq data obtained from COVID-19-positive patients and COVID-19-negative donors. RNA-seq data were collected for the purpose of identification of potential biomarkers associated with a different course of the disease. Here, the first datasets of 96 samples were analyzed to validate the methods. The aim of this publication is to report pilot results. In search of potential biomarkers associated with different disease severity, we performed differential expression analysis of human transcriptome, focusing on COVID-19 positivity and symptom severity. Since we detected plenty of potential biomarkers, we performed KEGG enrichment analysis to get better view of altered pathways. Results show, that affected were pathways related to immune processes and response to infection, also multiple signaling pathways, while most of them were also reported to be influenced by SARS-CoV-2 infection in previous studies.
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
,
,
,
*
