Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

MALDI-TOF MS KPC Direct Detection from Patients’ Positive Blood Culture Bottles, Short-term Cultures and Colonies at the Hospital

Agustina Costa
,
Roque Arnulfo Figueroa-Espinosa
ORCID logo ,
Jerson Andrés Martínez
,
Liliana Fernández-Canigia
,
María Ivana Maldonado
,
Silvina Bergese
,
Ana Schneider
,
Carlos Vay
,
Carlos Hernán Rodriguez
,
Marcela Nastro
,
Gabriel Osvaldo Gutkind
*,# ,
José Alejandro Di Conza
*,#
#
Both authors have contributed equally to this manuscript.
Version 1 : Received: 29 May 2023 / Approved: 31 May 2023 / Online: 31 May 2023 (10:50:42 CEST)

A peer-reviewed article of this Preprint also exists.

Costa, A.; Figueroa-Espinosa, R.; Martínez, J.A.; Fernández-Canigia, L.; Maldonado, M.I.; Bergese, S.A.; Schneider, A.E.; Vay, C.; Rodriguez, C.H.; Nastro, M.; Gutkind, G.O.; Di Conza, J.A. MALDI-TOF MS-Based KPC Direct Detection from Patients’ Positive Blood Culture Bottles, Short-Term Cultures, and Colonies at the Hospital. Pathogens 2023, 12, 865. Costa, A.; Figueroa-Espinosa, R.; Martínez, J.A.; Fernández-Canigia, L.; Maldonado, M.I.; Bergese, S.A.; Schneider, A.E.; Vay, C.; Rodriguez, C.H.; Nastro, M.; Gutkind, G.O.; Di Conza, J.A. MALDI-TOF MS-Based KPC Direct Detection from Patients’ Positive Blood Culture Bottles, Short-Term Cultures, and Colonies at the Hospital. Pathogens 2023, 12, 865.

Abstract

Carbapenemase resistance in Enterobacterales is a global public health problem and rapid and effective methods to detect resistance mechanisms are needed urgently. Our aim was to evaluate the performance of a MALDI-TOF MS based KPC detection protocol from patients’ positive blood cultures, short-term cultures and colonies at health care settings. Bacterial identification and KPC detection were achieved after protein extraction with organic solvents and target spot loading with suitable organic matrices. Confirmation of KPC production was performed by susceptibility tests, blaKPC amplification by PCR and sequencing. KPC direct detection (KPC-peak at approximately 28.681 Da) from patients’ positive blood cultures, short-term cultures and colonies, once bacterial identification was achieved, showed an overall sensibility and specificity of 100% (CI95: [95%,100%] and CI95: [99%, 100%], respectively). Concordance between hospital routine bacterial identification protocol and identification with this new methodology from the same extract used for KPC detection was ≥92%. This study represents the pioneering effort to directly detect KPC using MALD-TOF MS technology, conducted on patient-derived samples obtained at the hospitals for validation purposes, in a multi-resistance global context that requires concrete actions to preserve available therapeutic options and reduce the spread of antibiotic resistance markers.

Keywords

KPC; blood culture; short-term culture; MALDI-TOF MS

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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