Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Effects of Different Freezing Rate and Frozen Storage Temperature on the Quality of Large-mouth Bass (Micropterus salmoides)

Version 1 : Received: 23 May 2023 / Approved: 29 May 2023 / Online: 29 May 2023 (12:40:14 CEST)

A peer-reviewed article of this Preprint also exists.

Bao, Y.; Zhang, Y.; Xu, W. Effects of Different Freezing Rate and Frozen Storage Temperature on the Quality of Large-Mouth Bass (Micropterus salmoides). Molecules 2023, 28, 5432. Bao, Y.; Zhang, Y.; Xu, W. Effects of Different Freezing Rate and Frozen Storage Temperature on the Quality of Large-Mouth Bass (Micropterus salmoides). Molecules 2023, 28, 5432.

Abstract

In order to clarify the individual role of freezing and frozen storage on the quality of fish, fillets of large-mouth bass (Micropterus salmoides) were subjected to different freezing rates (freezing with -18 °C (A), -60 °C (B), -60 °C with forced air circulation at 2 m/s (C), respectively) followed by frozen storage at -18 °C for 30 and 90 days. Another 2 groups were frozen with -60 °C, followed by storage at -40 °C (D) and -60 °C (E), respectively. Results showed that water-holding and TVBN were mainly affected by storage time. No significant changes were found in free thiol content among treatments. A greater freezing rate and lower storage temperature generally led to lower TBARS. GC×GC-TOFMS revealed a total of 66 volatile compounds which were related to lipid oxidation. PLS-DA showed that fresh samples were separated from the frozen-thawed ones, and fillets in group D & E were relatively close to fresh fillets in the composition of oxidation-related volatiles. In conclusion, freezing rate and storage temperature had significant impact on lipid oxidation and protein denaturation in the fillets of large-mouth bass, while protein oxidation was more affected by freezing rate.

Keywords

Freezing; protein oxidation; lipid oxidation; water-holding; GC×GC-TOFMS

Subject

Biology and Life Sciences, Food Science and Technology

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