Version 1
: Received: 26 May 2023 / Approved: 29 May 2023 / Online: 29 May 2023 (04:28:28 CEST)
How to cite:
Mishra, S.; Sharma, P.; Singh, R.; Ahlawat, O.P.; Singh, G. Identification and Expression Analysis of Key Gene Families in RNA Silencing and Their Responses to Heat Stress in Wheat (Triticum aestivum). Preprints2023, 2023051961. https://doi.org/10.20944/preprints202305.1961.v1
Mishra, S.; Sharma, P.; Singh, R.; Ahlawat, O.P.; Singh, G. Identification and Expression Analysis of Key Gene Families in RNA Silencing and Their Responses to Heat Stress in Wheat (Triticum aestivum). Preprints 2023, 2023051961. https://doi.org/10.20944/preprints202305.1961.v1
Mishra, S.; Sharma, P.; Singh, R.; Ahlawat, O.P.; Singh, G. Identification and Expression Analysis of Key Gene Families in RNA Silencing and Their Responses to Heat Stress in Wheat (Triticum aestivum). Preprints2023, 2023051961. https://doi.org/10.20944/preprints202305.1961.v1
APA Style
Mishra, S., Sharma, P., Singh, R., Ahlawat, O.P., & Singh, G. (2023). Identification and Expression Analysis of Key Gene Families in RNA Silencing and Their Responses to Heat Stress in Wheat (<em>Triticum aestivum</em>). Preprints. https://doi.org/10.20944/preprints202305.1961.v1
Chicago/Turabian Style
Mishra, S., Om P Ahlawat and G Singh. 2023 "Identification and Expression Analysis of Key Gene Families in RNA Silencing and Their Responses to Heat Stress in Wheat (<em>Triticum aestivum</em>)" Preprints. https://doi.org/10.20944/preprints202305.1961.v1
Abstract
RNA interference is a highly conserved process in which non-coding small RNAs (sRNA) modulate gene expression at the post-transcriptional level influencing plant growth and development. Dicer-like (DCL), Argonaute (AGO) and RNA-dependent RNA polymerase (RDR) are the core elements involved in gene silencing and their gene families have been explored in many plants. However, these genes and their response to abiotic stresses have not yet been well in wheat. In this study, 82 AGO, 31 DCL, and 31 RDR genes were identified and phylogenetic analysis of these proteins showed that clustered into ten, four and four clades respectively. RNA-seq analysis revealed constitutive expression of AGO1, AGO9, and DCL2 family expression analysis in tissues under normal and stress conditions, whereas RDR1 which is known to engage in siRNA biogenesis showed higher expression levels in wheat leaf tissues. Our findings build the foundation for comparative genomics analyses of RNA silencing elements in cereal crops, as well as new insights into the functional complexity of RNA silencing in wheat stress responses, which is critical for understanding the processes underlying wheat stress responses.
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
