The Combination of Binding-Avidity of Ovomucoid-Specific IgE Antibody and Specific IgG4 Antibody Can Predict Positive Outcome of Oral Food Challenge during Stepwise Slow Oral Immunotherapy in Children with Hen’s Egg Allergy

Shoichiro Taniuchi; Rika Sakai; Takashi Nishida; Meguru Goma; Masatoshi Mitomori; Aya Imaide; Masahiro Enomoto; Masamitsu Nishino; Yo Okizuka; Hiroshi Kido

doi:10.20944/preprints202305.1836.v2

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preprints.org > biology and life sciences > food science and technology > doi: 10.20944/preprints202305.1836.v2

Preprint Article Version 2 Preserved in Portico This version is not peer-reviewed

The Combination of Binding-Avidity of Ovomucoid-Specific IgE Antibody and Specific IgG4 Antibody Can Predict Positive Outcome of Oral Food Challenge during Stepwise Slow Oral Immunotherapy in Children with Hen’s Egg Allergy

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Version 1 : Received: 25 May 2023 / Approved: 26 May 2023 / Online: 26 May 2023 (04:06:33 CEST)
Version 2 : Received: 26 May 2023 / Approved: 26 May 2023 / Online: 26 May 2023 (07:25:05 CEST)
Version 3 : Received: 26 May 2023 / Approved: 31 May 2023 / Online: 31 May 2023 (02:28:20 CEST)

A peer-reviewed article of this Preprint also exists.

Taniuchi, S.; Sakai, R.; Nishida, T.; Goma, M.; Mitomori, M.; Imaide, A.; Enomoto, M.; Nishino, M.; Okizuka, Y.; Kido, H. The Combination of Binding Avidity of Ovomucoid-Specific IgE Antibody and Specific IgG4 Antibody Can Predict Positive Outcomes of Oral Food Challenges during Stepwise Slow Oral Immunotherapy in Children with Hen’s Egg Allergy. Nutrients 2023, 15, 2770. Taniuchi, S.; Sakai, R.; Nishida, T.; Goma, M.; Mitomori, M.; Imaide, A.; Enomoto, M.; Nishino, M.; Okizuka, Y.; Kido, H. The Combination of Binding Avidity of Ovomucoid-Specific IgE Antibody and Specific IgG4 Antibody Can Predict Positive Outcomes of Oral Food Challenges during Stepwise Slow Oral Immunotherapy in Children with Hen’s Egg Allergy. Nutrients 2023, 15, 2770.

Abstract

To increase the prediction accuracy of positive oral food challenge (OFC) outcome during stepwise slow oral immunotherapy (SS-OIT) in children with hen’s egg (HE) allergy, we evaluated the predictive value of the combination of antigen-specific IgE (sIgE) with antigen binding-avidity and sIgG4 values. Sixty-three children with HE allergy undergoing SS-OIT were subjected to repeated OFC with HE. We measured the ovomucoid (OVM)-sIgE by ImmunoCAP or densely carboxylated protein (DCP) microarray, sIgG4 by DCP microarray, and the binding-avidity of OVM-sIgE defined as the level of 1/ IC50 (nM) measured by competitive binding inhibition assays. The OFC was positive in 37 (59%) patients undergoing SS-OIT. Significant differences in DCP-OVM-sIgE, CAP-OVM-sIgE, I/ IC50, DCP-OVM-sIgG4, the multiplication products of DCP-OVM-sIgE and binding-avidity of DCP-OVM-sIgE (DCP-OVM-sIgE/IC50) and DCP-OVM-sIgE/sIgG4 were compared between the negative and positive groups (p<0.01). Among them, the variable with greatest area under the receiver operating characteristic curve was DCP-OVM-sIgE/IC50 (0.84), followed by DCP-OVM-sIgE/sIgG4 (0.81). DCP-OVM-sIgE/IC50 and DCP-OVM-sIgE/sIgG4 are potentially useful markers for the prediction of positive OFC during HE-SS-OIT and may allow proper evaluation of the current allergic status in the healing process during HE-SS-OIT

Keywords

oral immunotherapy; hen’s egg allergy; IgE binding-avidity; ovomucoid specific IgE; ovomucoid specific IgG4; oral food challenge

Subject

Biology and Life Sciences, Food Science and Technology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Comments (1)

Comment 1

Received: 26 May 2023
Commenter: Shoihciro Taniuchi
Commenter's Conflict of Interests: Author

Comment: I revised few word in the previous article as correct words and also found that Fig .3 was incomplete and missing in the aruticle in your website . I would like to change revised version for review.

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