Version 1
: Received: 24 May 2023 / Approved: 26 May 2023 / Online: 26 May 2023 (02:46:45 CEST)
How to cite:
André, R.; Pacheco, R.; Santos, H.M.; Serralheiro, M. Omic Studies on the Effect of Fucus vesiculosus on Differentiated Caco-2 Cells: A Potential Functional Food. Preprints2023, 2023051819. https://doi.org/10.20944/preprints202305.1819.v1
André, R.; Pacheco, R.; Santos, H.M.; Serralheiro, M. Omic Studies on the Effect of Fucus vesiculosus on Differentiated Caco-2 Cells: A Potential Functional Food. Preprints 2023, 2023051819. https://doi.org/10.20944/preprints202305.1819.v1
André, R.; Pacheco, R.; Santos, H.M.; Serralheiro, M. Omic Studies on the Effect of Fucus vesiculosus on Differentiated Caco-2 Cells: A Potential Functional Food. Preprints2023, 2023051819. https://doi.org/10.20944/preprints202305.1819.v1
APA Style
André, R., Pacheco, R., Santos, H.M., & Serralheiro, M. (2023). Omic Studies on the Effect of <em>Fucus vesiculosus</em> on Differentiated Caco-2 Cells: A Potential Functional Food. Preprints. https://doi.org/10.20944/preprints202305.1819.v1
Chicago/Turabian Style
André, R., Hugo M. Santos and Maria-Luísa Serralheiro. 2023 "Omic Studies on the Effect of <em>Fucus vesiculosus</em> on Differentiated Caco-2 Cells: A Potential Functional Food" Preprints. https://doi.org/10.20944/preprints202305.1819.v1
Abstract
High blood cholesterol levels are one of the main risks for atherosclerotic disease. A purified aqueous extract of Fucus vesiculosus, characterized as rich in phlorotannins and peptides, was described as having the capacity to inhibit cholesterol biosynthesis and intestinal absorption. In this work, the effect of this extract on intestinal cells metabolites and proteins was analysed, aiming to expand the knowledge about its mode of action targeting cholesterol metabolism, in particular exogenous cholesterol absorption and transport. Caco-2 cells differentiated into enterocytes were exposed to the purified aqueous extract of F. vesiculosus and analysed by undirected metabolomics and proteomics. The results of the metabolomic analysis showed only statistically significant differences in glutathione content of the cells exposed to the extract relatively to the control cells, with a decreased glutathione expression in exposed cells. The proteomic analysis showed an increased expression for cells exposed to the extract of NPC1, an important protein known to be involved in cholesterol transport. To extent of our knowledge this is the first study using untargeted metabolomics and proteomic analysis to study the effect of F. vesiculosus on differentiated Caco-2 cells, which aims to provide some insight about the molecular mechanism of extract compounds on intestinal cells.
Keywords
Fucus vesiculosus; proteomics; metabolomics; NPC1; Caco-2 cell line
Subject
Biology and Life Sciences, Life Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
