Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Recognition Mechanisms of Hemoglobin Particles by Monocytes – CD163 May Just Be One

Jonathan-Gabriel Nimz
,
Pichayut Rerkshanandana
,
Chiraphat Kloypan
ORCID logo ,
Ulrich Kalus
,
Saranya Chaiwaree
,
Axel Pruß
,
Radostina Georgieva
ORCID logo ,
Yu Xiong
ORCID logo ,
Hans Bäumler
* ORCID logo
Version 1 : Received: 23 May 2023 / Approved: 24 May 2023 / Online: 24 May 2023 (04:04:06 CEST)

How to cite: Nimz, J.; Rerkshanandana, P.; Kloypan, C.; Kalus, U.; Chaiwaree, S.; Pruß, A.; Georgieva, R.; Xiong, Y.; Bäumler, H. Recognition Mechanisms of Hemoglobin Particles by Monocytes – CD163 May Just Be One. Preprints 2023, 2023051681. https://doi.org/10.20944/preprints202305.1681.v1 Nimz, J.; Rerkshanandana, P.; Kloypan, C.; Kalus, U.; Chaiwaree, S.; Pruß, A.; Georgieva, R.; Xiong, Y.; Bäumler, H. Recognition Mechanisms of Hemoglobin Particles by Monocytes – CD163 May Just Be One. Preprints 2023, 2023051681. https://doi.org/10.20944/preprints202305.1681.v1

Abstract

Hemoglobin based oxygen carriers (HBOC) as blood substitutes are one of the great hopes of modern transfusion and emergency medicine. After the major safety-relevant challenges of the last decades seem to be largely overcome, current developments have in common that they are affected by degradation and excretion at an early stage in test organisms. Several possible mechanisms that may be responsible for this are discussed in the literature. One of them is CD163, the receptor of the complex of haptoglobin (Hp) and hemoglobin (Hb). The receptor has been shown in various studies to have an affinity for Hb in the absence of Hp. Thus, it seems reasonable that CD163 could possibly also bind Hb within HBOC and cause phagocytosis of the particles. In this work we investigated the role of CD163 for the uptake of our HbMP (hemoglobin sub-micron particles) in monocytes and additionally screened for alternative ways of particle recognition by monocytes. In our experiments, blockade of CD163 by specific monoclonal antibody proved to partly inhibit HbMP-uptake by monocytes. It appears however, that several other phagocytosis pathways for HbMP might exist, independent of CD163 and also Hb.

Keywords

hemoglobin sub-micron particles; CD163; monocytes; scavenger receptors

Subject

Chemistry and Materials Science, Biomaterials

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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