Version 1
: Received: 19 May 2023 / Approved: 22 May 2023 / Online: 22 May 2023 (11:19:23 CEST)
Version 2
: Received: 27 May 2023 / Approved: 29 May 2023 / Online: 29 May 2023 (14:29:16 CEST)
Kumar, J., Tyagi, P., Singh, R., Saini, P., Sharma, D., & Maras, J. (2023). Thiourea Derivatives Restore Dysfunctional Mitochondria in Chronic Hepatitis B Infection. Reviewed, 11. https://doi.org/10.35248/0974-276X.23.16.652
Kumar, J., Tyagi, P., Singh, R., Saini, P., Sharma, D., & Maras, J. (2023). Thiourea Derivatives Restore Dysfunctional Mitochondria in Chronic Hepatitis B Infection. Reviewed, 11. https://doi.org/10.35248/0974-276X.23.16.652
Kumar, J., Tyagi, P., Singh, R., Saini, P., Sharma, D., & Maras, J. (2023). Thiourea Derivatives Restore Dysfunctional Mitochondria in Chronic Hepatitis B Infection. Reviewed, 11. https://doi.org/10.35248/0974-276X.23.16.652
Kumar, J., Tyagi, P., Singh, R., Saini, P., Sharma, D., & Maras, J. (2023). Thiourea Derivatives Restore Dysfunctional Mitochondria in Chronic Hepatitis B Infection. Reviewed, 11. https://doi.org/10.35248/0974-276X.23.16.652
Abstract
Chronic hepatitis B (CHB) infection and the Hepatitis B virus X protein (HBx) are major risk factors associated with hepatocellular carcinoma (HCC). In CHB infection, HBx induces mitochondrial dysfunction, exhaustion and impaired function in hepatocytes. Restoring hepatocyte health along with reduction in virus replication could be an ideal treatment for CHB. Thiourea derivatives are well known for their antiviral property though their effect on mitochondrial and/ or hepatocyte health remains obscure. This study focus on the repurposing of thiourea derivatives (DSA-00, DSA-02, and DSA-09) on hepatocyte replenishment. HepG2.2.15 cells were treated with thiourea derivatives, alongside Entecavir (ETV). The proteomics analysis showed both DSA-00 and ETV were enriched with proteins associated with antiviral responses. In addition, DSA-00 additionally showed increase in proteins linked to mitochondrial response. Whereas DSA-02 exhibited association with innate immune system and citric acid cycle and DSA-09 displayed pathways similar to DSA-00 and ETV. Treated groups exhibited enhanced bio-energetic and antiviral response as compared to the untreated group. FACS analysis revealed the restoration of exhausted hepatocytes by thiourea derivatives through targeting mitochondria. Our findings suggest that thiourea derivatives hold potential as a novel therapeutic agent that seems to restore mitochondrial health along with anti-viral response in CHB.
Keywords
Antiviral; Thiourea derivatives; Hepatitis B Virus; Chronic hepatitis B infection; Mitochondria dysfunction; Exhausted hepatocytes
Subject
Biology and Life Sciences, Virology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Commenter: Jitendra Kumar
Commenter's Conflict of Interests: Author
Revised abstract
revised result
modifed the figure