Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Isolation and Evaluation of the Antagonistic Activity of Cinidium Officinale Rhizosphere Bacteria Against Phytopathogenic Fungi (Fusarium solani)

Seok Hui Lee
ORCID logo ,
Su Hong Jeon
,
Jun Young Park
,
Dae Sol Kim
ORCID logo ,
Ji Ah Kim
,
Hui Yeong Jeong
,
Jun Won Kang
*
Version 1 : Received: 18 May 2023 / Approved: 19 May 2023 / Online: 19 May 2023 (03:48:36 CEST)

A peer-reviewed article of this Preprint also exists.

Lee, S.H.; Jeon, S.H.; Park, J.Y.; Kim, D.S.; Kim, J.A.; Jeong, H.Y.; Kang, J.W. Isolation and Evaluation of the Antagonistic Activity of Cnidium officinale Rhizosphere Bacteria against Phytopathogenic fungi (Fusarium solani). Microorganisms 2023, 11, 1555. Lee, S.H.; Jeon, S.H.; Park, J.Y.; Kim, D.S.; Kim, J.A.; Jeong, H.Y.; Kang, J.W. Isolation and Evaluation of the Antagonistic Activity of Cnidium officinale Rhizosphere Bacteria against Phytopathogenic fungi (Fusarium solani). Microorganisms 2023, 11, 1555.

Abstract

: Cnidium officinale Makino, a perennial crop in the Umbeliperae family, is harvested in the summer for medicinal purposes. The average temperature in Korea has recently increased, and consequently, the area used to cultivate C. officinale has been reduced, and plant disease and soil sickness have increased. In this study, rhizosphere bacteria were isolated from C. officinale, and their antagonistic activity evaluated against Fusarium solani, which is a major issue during repeated cultivation. Twenty-eight strains were isolated from the root and rhizosphere soil, of which seven showed antagonistic activity against F. solani and were further investigated. An in planta test found that the dead rate of shoots was significantly lower in the group inoculated with PT1 isolated from the root tissue and that the fresh and dry weights were also higher, when compared with the other groups. PT1 was thus considered to have antagonistic activity against F. solani. The 16S rRNA analysis identified the PT1 as Leclercia adecarboxylata, and downstream studies confirmed the production of enzymes related to antagonism, such as siderophore and N-acetyl-β-glucosaminidase. The phosphorous solubilizing ability and secretion of related enzymes were also confirmed. The results indicate that PT1 could be utilized as a PGPR to help control fusarium wilt.

Keywords

PGPR; Cnidium officinale; Leclercia adecarboxylata; Bacillus; Fusarium solani; Fusarium wilt

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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