Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

In Vitro Activity of Ozone/Oxygen Gaseous Mixture against Caprine Herpesvirus Type 1 (CpHV-1) Strain Isolated from Vaginitis in Goat

Edoardo Lillo
ORCID logo ,
Francesco Pellegrini
ORCID logo ,
Annalisa Rizzo
* ORCID logo ,
Gianvito Lanave
ORCID logo ,
Claudia Zizzadoro
ORCID logo ,
Vincenzo Cicirelli
* ,
Cristiana Catella
ORCID logo ,
Michele Losurdo
,
Vito Martella
ORCID logo ,
Maria Tempesta
ORCID logo ,
Michele Camero
Version 1 : Received: 18 May 2023 / Approved: 18 May 2023 / Online: 18 May 2023 (10:27:21 CEST)

A peer-reviewed article of this Preprint also exists.

Lillo, E.; Pellegrini, F.; Rizzo, A.; Lanave, G.; Zizzadoro, C.; Cicirelli, V.; Catella, C.; Losurdo, M.; Martella, V.; Tempesta, M.; Camero, M. In Vitro Activity of Ozone/Oxygen Gaseous Mixture Against a Caprine Herpesvirus Type 1 Strain Isolated from a Goat with Vaginitis. Animals 2023, 13, 1920. Lillo, E.; Pellegrini, F.; Rizzo, A.; Lanave, G.; Zizzadoro, C.; Cicirelli, V.; Catella, C.; Losurdo, M.; Martella, V.; Tempesta, M.; Camero, M. In Vitro Activity of Ozone/Oxygen Gaseous Mixture Against a Caprine Herpesvirus Type 1 Strain Isolated from a Goat with Vaginitis. Animals 2023, 13, 1920.

Abstract

Alphaherpesviruses cause genital lesions and reproductive failure in both humans and animals. Their control is mainly based on prevention by hygienic prophylactic measures, due to the ab-sence of vaccines and limitations of antiviral drug therapy. Ozone is an oxidating gas showing a strong microbicidal activity on bacteria, fungi, viruses, and protozoa. The present study assessed the in vitro virucidal and antiviral activity of ozone against Caprine herpesvirus type 1. Virucidal activity of a gaseous mixture containing O3 at 20 and 50 μg /mL was assessed against the virus for different contact times (30 s, 60s, 90s, 120s, 180s and 300s). Antiviral activity of a gaseous mix-ture containing O3 at 20 and 50 μg /mL was evaluated against the virus to for 30s and 60s. Ozone displayed significant virucidal activity when used at all the tested concentrations whilst signifi-cant antiviral activity was observed using ozone at 50 μg/ml. The gaseous mixture, tested in the present study, showed virucidal and antiviral activity against CpHV-1 with a dose- and a time-contact -dependent fashion. Ozone therapy could be evaluated in vivo for the treatment of CpHV-1-induced genital lesions in goats, through topical applications.

Keywords

Caprine herpesvirus; virucidal activity; Ozone; in vitro; antiviral activity

Subject

Medicine and Pharmacology, Veterinary Medicine

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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