Yang, L.; Zhao, J.; Fan, S.; Liao, J.; Chen, Y.; Wang, Y. Effect of Frost on the Different Metabolites of Two Mulberry (Morus nigra L. and Morus alba L.) Leaves. Molecules2023, 28, 4718.
Yang, L.; Zhao, J.; Fan, S.; Liao, J.; Chen, Y.; Wang, Y. Effect of Frost on the Different Metabolites of Two Mulberry (Morus nigra L. and Morus alba L.) Leaves. Molecules 2023, 28, 4718.
Yang, L.; Zhao, J.; Fan, S.; Liao, J.; Chen, Y.; Wang, Y. Effect of Frost on the Different Metabolites of Two Mulberry (Morus nigra L. and Morus alba L.) Leaves. Molecules2023, 28, 4718.
Yang, L.; Zhao, J.; Fan, S.; Liao, J.; Chen, Y.; Wang, Y. Effect of Frost on the Different Metabolites of Two Mulberry (Morus nigra L. and Morus alba L.) Leaves. Molecules 2023, 28, 4718.
Abstract
Mulberry leaves are a classic herb commonly used in traditional Chinese medicine, and experience since ancient times has shown that leaves collected after frost have better medicinal properties. However, the characteristics of the changes of critical metabolic components in mulberry leaves, especially Morus nigra L., are still unknown. To further study the influence of harvesting time on the dynamic changes of metabolites, especially DNJ compounds and flavonoids, in mulberry leaves after frost. We used metabolomics technology to select 100+ metabolites as indexes to reflect the dynamic changes of metabolites in mulberry leaves comprehensively. The results showed significant differences in the effects of defrosting on the accumulation of metabolites in the leaves of Morus nigra L. and Morus alba L. The content of DNJ in the leaves of Morus nigra L. showed a decreasing trend after the frost, while flavonoids reached the peak of the second frost. For Morus alba L, DNJ showed an increasing trend after frost, especially reaching its peak one day after the second frost. Flavonoids mainly peak in the week before the frost. In addition, by analyzing the influence of picking time on metabolite accumulation in two mulberry leaves, it was concluded that higher DNJ alkaloids and flavonoids would be obtained in mulberry leaves collected in the morning. This study also provides scientific guidance for the optimum harvesting time of mulberry leaves.
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