Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Under the Hood: Understanding the Features of Mucin in Pseudomyxoma Peritonei

Pedro Villarejo-Campos
* ,
Mariano García-Arranz
ORCID logo ,
Siyuan Qian
,
Santos Jiménez de los Galanes
,
Victor Dominguez-Prieto
,
Juan Felipe Vélez Pinto
ORCID logo ,
Ismael Guijo Castellano
,
Montiel Jiménez-Fuertes
,
Hector Guadalajara
,
Damian Garcia-Olmo
ORCID logo
Version 1 : Received: 16 May 2023 / Approved: 17 May 2023 / Online: 17 May 2023 (04:33:59 CEST)

A peer-reviewed article of this Preprint also exists.

Villarejo-Campos, P.; García-Arranz, M.; Qian, S.; Jiménez de los Galanes, S.; Domínguez-Prieto, V.; Vélez-Pinto, J.F.; Guijo Castellano, I.; Jiménez-Fuertes, M.; Guadalajara, H.; García-Olmo, D. Under the Hood: Understanding the Features of Mucin in Pseudomyxoma Peritonei. J. Clin. Med. 2023, 12, 4007. Villarejo-Campos, P.; García-Arranz, M.; Qian, S.; Jiménez de los Galanes, S.; Domínguez-Prieto, V.; Vélez-Pinto, J.F.; Guijo Castellano, I.; Jiménez-Fuertes, M.; Guadalajara, H.; García-Olmo, D. Under the Hood: Understanding the Features of Mucin in Pseudomyxoma Peritonei. J. Clin. Med. 2023, 12, 4007.

Abstract

Pseudomyxoma peritonei (PMP) is a rare malignant growth characterized by the production of mucin and the potential for peritoneal relapse. This study aimed to investigate the immunohistochemical and biological characteristics of mucin in patients with cellular and acellular PMP. We prospectively analyzed mucin specimens obtained from our patient cohort and described the composition and type of mucin present in each sample. A metagenomic analysis of the samples was performed to investigate the bacterial composition of the PMP microbiome. Secreted mucins 2 and 5AC and membrane-associated mucin-1 were the primary components of mucin in both cellular and acellular tumor specimens. The metagenomic study revealed a predominance of the phylum Proteobacteria and the genus Pseudomonas. Notably, Pseudomonas plecoglossicida, a species not previously reported in the human microbiome, was found to be the most abundant organism in the mucin of pseudomyxoma peritonei. Our findings suggest that the presence of MUC-2 and mucin colonization by Pseudomonas is a characteristic feature of both cellular and acellular disease. These results may have significant implications for the diagnosis and treatment of this rare entity.

Keywords

pseudomyxoma peritonei; mucin; MUC-2; microbiome; pseudomonas.

Subject

Medicine and Pharmacology, Surgery

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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