Mantareva, V.; Iliev, I.; Sulikovska, I.; Durmuş, M.; Genova, T. Collagen Hydrolysate Effects on Photodynamic Efficiency of Gallium (III) Phthalocyanine on Pigmented Melanoma Cells. Gels 2023, 9, 475, doi:10.3390/gels9060475.
Mantareva, V.; Iliev, I.; Sulikovska, I.; Durmuş, M.; Genova, T. Collagen Hydrolysate Effects on Photodynamic Efficiency of Gallium (III) Phthalocyanine on Pigmented Melanoma Cells. Gels 2023, 9, 475, doi:10.3390/gels9060475.
Mantareva, V.; Iliev, I.; Sulikovska, I.; Durmuş, M.; Genova, T. Collagen Hydrolysate Effects on Photodynamic Efficiency of Gallium (III) Phthalocyanine on Pigmented Melanoma Cells. Gels 2023, 9, 475, doi:10.3390/gels9060475.
Mantareva, V.; Iliev, I.; Sulikovska, I.; Durmuş, M.; Genova, T. Collagen Hydrolysate Effects on Photodynamic Efficiency of Gallium (III) Phthalocyanine on Pigmented Melanoma Cells. Gels 2023, 9, 475, doi:10.3390/gels9060475.
Abstract
Collagen has been accepted in drug delivery structures due to its biocompatible chemical, physicochemical and curative actions. The study aims to evaluate the effects of a bovine collagen hydrolysate (Clg) on the main photophysical and photodynamic properties of a new gallium (III) phthalocyanine (GaPc). The absorbance of GaPc and the conjugate GaPc-Clg showed a decrease of the intensive Q-band (681 nm) and a blue shift of the maximum (678 nm) with loss of UV-band (354 nm) for 40 mg/mL Clg. The fluorescence of GaPc was evaluated with strong emission peak at 694 nm (exc: 615 nm) which was lower for GaPc-Clg (691 nm). The quantum yield of 0.23 was obtained for GaPc which was reduced for GaPc-Clg (0.012). Photo- and dark cytotoxicity was studied by NRU-assay on pigmented melanoma cells (SH-4) and normal cell lines (BJ and HaCaT). The results suggested a slight reduction of cytotoxicity for the conjugate but almost double for the selectivity index (SI: 0.71 vs. 1.49 for HaCaT cells). The study showed that bovine collagen hydrolysate can lower the PDT activity of a powerful photosensitizer with phototherapeutic index: 819 to 360, but also minimized its dark toxicity as a positive effect in the photodynamic treatment.
Keywords
bovine collagen hydrolysate; gallium phthalocyanine; pigmented melanoma; normal keratocytes and fibroblasts; mouse embryonal fibroblasts; photodynamic therapy
Subject
Medicine and Pharmacology, Medicine and Pharmacology
Copyright:
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