Ntemafack, A.; Dzelamonyuy, A.; Nchinda, G.; Bopda Waffo, A. Evolutionary Qβ Phage Displayed Nanotag Library and Peptides for Biosensing. Viruses2023, 15, 1414.
Ntemafack, A.; Dzelamonyuy, A.; Nchinda, G.; Bopda Waffo, A. Evolutionary Qβ Phage Displayed Nanotag Library and Peptides for Biosensing. Viruses 2023, 15, 1414.
Ntemafack, A.; Dzelamonyuy, A.; Nchinda, G.; Bopda Waffo, A. Evolutionary Qβ Phage Displayed Nanotag Library and Peptides for Biosensing. Viruses2023, 15, 1414.
Ntemafack, A.; Dzelamonyuy, A.; Nchinda, G.; Bopda Waffo, A. Evolutionary Qβ Phage Displayed Nanotag Library and Peptides for Biosensing. Viruses 2023, 15, 1414.
Abstract
We introduced a novel biotin-binding peptide for sensing biotin, biotinylated proteins, and nu-cleotides. From a 15-mer library displayed on the RNA coliphage Qβ, a 15-amino acid long peptide (HGHGWQIPVWPWGQG) hereby referred to as nanotag was identified to selectively bind biotin. The targets selection was achieved through panning with elution by infection. The selected peptide was tested as a transducer for an immunogenic epitope of the foot-and-mouth disease virus (FMDV) on Qβ phage platform separated by a linker. The biotin-tag showed no significant in-fluence on the affinity of the epitope to its cognate antibody (SD6). The nanotag bound biotin selectively when fused either to the C- or N-terminus of the epitope. The epitope would not bind or recognize SD6 while positioned at the N-terminus of the nanotag. Additionally, the biotin com-peted linearly with the SD6 antibody in a competitive ELISA. Competition assays using the se-lected recombinant phage itself as a probe, or transducer, enable the operationalization of this technology as a biosensor toolkit to sense and quantify SD6 analyte. Herein, the published Strep II nanotag (DVEWLDERVPLVET) was used as a control and has similar functionalities to our pro-posed novel biotin-tag thereby providing a new platform for developing devices for diagnostic purposes.
Keywords
Biosensor; Biotin-tag; transducer
Subject
Biology and Life Sciences, Virology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
