Preprint Essay Version 1 Preserved in Portico This version is not peer-reviewed

The Plant-Derived Lactic Acid Bacteria Increases the Cell Death of Human Colon Cancer Cell via Modulating the Apoptosis and Ferroptosis

Yuhan Huang
ORCID logo ,
Yanyang Wu
ORCID logo ,
Yong Li
ORCID logo ,
Ziyu Nie
,
Shiyu Wang
,
Zengguang Wang
,
Ding Ma
,
Fangming Deng
* ,
Lingyan Zhao
*
Version 1 : Received: 15 May 2023 / Approved: 15 May 2023 / Online: 15 May 2023 (10:22:56 CEST)
Version 2 : Received: 16 May 2023 / Approved: 16 May 2023 / Online: 16 May 2023 (10:38:21 CEST)

How to cite: Huang, Y.; Wu, Y.; Li, Y.; Nie, Z.; Wang, S.; Wang, Z.; Ma, D.; Deng, F.; Zhao, L. The Plant-Derived Lactic Acid Bacteria Increases the Cell Death of Human Colon Cancer Cell via Modulating the Apoptosis and Ferroptosis. Preprints 2023, 2023051019. https://doi.org/10.20944/preprints202305.1019.v1 Huang, Y.; Wu, Y.; Li, Y.; Nie, Z.; Wang, S.; Wang, Z.; Ma, D.; Deng, F.; Zhao, L. The Plant-Derived Lactic Acid Bacteria Increases the Cell Death of Human Colon Cancer Cell via Modulating the Apoptosis and Ferroptosis. Preprints 2023, 2023051019. https://doi.org/10.20944/preprints202305.1019.v1

Abstract

Colon cancer is a common malignancy of digestive tract and has high incidence and mortality worldwide. As a normal flora in the human gastrointestinal tract, lactic acid bacteria have been proved to have health benefits, such as immune-regulation, anti-infection, and anti-tumor. In the present study, the inhibitory effects of 19 heat-inactivated plant-derived lactic acid bacteria on human colon cancer cell HCT116 were investigated. The results showed that heat-inactivated plant-derived lactic acid bacteria strain 729 had the strongest inhibitory effect on HCT116 cells among all tested bacteria. Treatment with heat-inactivated bacteria strain 729 could inhibit the proliferation of HT-29 cells in dose-dependent manner, with 54.52% inhibition at a final concentration of 1×108 CFU/mL. Moreover, the heat-inactivated bacteria strain 729 could block the cell cycle, induce apoptosis, and promote cellular ferroptosis by downregulating the expression of GPX4 and xCT at protein level. According to the 16S rDNA sequencing, the plant-derived lactic acid bacteria strain 729 was Lactobacillus plantarum. Collectively, the results indicates that lactic acid bacteria strain 729 may be developed as special ingredients targeting colon cancer, but further in vivo studies are required.

Keywords

Colon cancer; Plant-derived lactic acid bacteria 729; Lactobacillus plantarum; Apoptosis; Ferroptosis

Subject

Biology and Life Sciences, Food Science and Technology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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