Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Evaluation of Magonia pubescens Extract against Phytopathogens: Searching for Eco-Friendly Crop Protection Products

Acácio R. A. Moraes
,
Samuel R. Sabina
,
Daniela G. Expósito
,
Cristina Giménez
ORCID logo ,
Guacimara Espinel
,
Grasiely F. Sousa
,
Lucienir P. Duarte
ORCID logo ,
Ignacio A. Jiménez
ORCID logo ,
Raimundo Cabrera
,
Isabel L. Bazzocchi
*
Version 1 : Received: 9 May 2023 / Approved: 12 May 2023 / Online: 12 May 2023 (13:32:35 CEST)

A peer-reviewed article of this Preprint also exists.

Moraes, A.R.A.; Sabina, S.R.; Expósito, D.G.; Giménez, C.; Espinel, G.; Sousa, G.F.; Duarte, L.P.; Jiménez, I.A.; Cabrera, R.; Bazzocchi, I.L. Evaluation of Magonia pubescens A. St.-Hill. Roots Extract against Phytopathogens: Searching for Eco-Friendly Crop Protection Products. Appl. Sci. 2023, 13, 6736. Moraes, A.R.A.; Sabina, S.R.; Expósito, D.G.; Giménez, C.; Espinel, G.; Sousa, G.F.; Duarte, L.P.; Jiménez, I.A.; Cabrera, R.; Bazzocchi, I.L. Evaluation of Magonia pubescens A. St.-Hill. Roots Extract against Phytopathogens: Searching for Eco-Friendly Crop Protection Products. Appl. Sci. 2023, 13, 6736.

Abstract

Botanical biopesticides have emerged as sustainable and eco-friendly alternatives to synthetic pesticides, whose indiscriminate use leads to several drawbacks to human and environment health. The present study reports on the evaluation of the roots extract from Magonia pubescens, species from the Cerrado (Brazilian savannah), on the phytopathogenic fungi, Alternaria alternata, Botrytis cinerea, and Fusarium oxysporum. In addition, its insect antifeedant effect was assayed against Chrysodeixis chalcites. Thus, an in vitro test-assay was used to determine the fungicide potential (percentage growth inhibition, % GI) of the ethanolic extract of this plant species, whereas a leaf-disk bioassay on the 5th instar larvae of C. chalcites was performed to evaluate the insecticide potential. The ethanolic extract was further fractionated by liquid-liquid extraction, using organic solvents of increasing polarity. The hexane/dichloromethane fraction exhibited a moderated potency, and similar to the ethanolic extract on the three assayed fungi (around % GI 30 at 1 mg/mL), whereas the n-butanol fraction showed a slight improvement of the fungicide effect against B. cinerea (% GI 39.18 at 1 mg/mL). Moreover, the etanolic extract exhibited a strong antifeedant activity, with a refusal rate (FR) higher than 90% in both choice and non-choice assays against C. chalcites, while the ethyl acetate and n-butanol fractions behaved as appetite suppressors. These results highlight M. pubescens as a promising source of biopesticides and deserve further investigations to optimize extraction procedures.

Keywords

Magonia pubescens; biopesticides; phytopathogenic fungi; Chrysodeixis chalcites

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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