Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Knocking Out OsNAC050 Expression Causes Low Temperature Tolerance in Rice by Regulating Photosynthesis and the Sucrose Metabolic Pathway

Bo Wang
ORCID logo ,
Yiheng Wang
ORCID logo ,
Likun Xie
,
Wancong Yu
,
Qingkuo Lan
,
Yong Wang
,
Chengbin Chen
* ,
Yong Zhang
* ORCID logo
Version 1 : Received: 8 May 2023 / Approved: 9 May 2023 / Online: 9 May 2023 (05:32:06 CEST)

A peer-reviewed article of this Preprint also exists.

Wang, B.; Wang, Y.; Xie, L.; Yu, W.; Lan, Q.; Wang, Y.; Chen, C.; Zhang, Y. Knocking out OsNAC050 Expression Causes Low-Temperature Tolerance in Rice by Regulating Photosynthesis and the Sucrose Metabolic Pathway. Agriculture 2023, 13, 1378. Wang, B.; Wang, Y.; Xie, L.; Yu, W.; Lan, Q.; Wang, Y.; Chen, C.; Zhang, Y. Knocking out OsNAC050 Expression Causes Low-Temperature Tolerance in Rice by Regulating Photosynthesis and the Sucrose Metabolic Pathway. Agriculture 2023, 13, 1378.

Abstract

Because rice is native to tropical and subtropical regions, it is generally more sensitive to cold stress compared to other cereals. Low temperature stress has become one of the most important conditions that affects the growth, development, and yield of rice. Plant NAC (NAM, ATAF1/2, and CUC) transcription factors are known to play significant regulatory roles in the stress response. In our research, we found that OsNAC050 (LOC_Os03g60080） is mainly expressed in leaves, and low temperature can further up-regulate its expression. OsNAC050 mutants created using CRISPR-Cas9 gene editing technology showed significantly enhanced tolerance to low temperature treatment. Detection of enzyme activities related to the redox pathway also showed that the mutants had stronger viability under low temperature stress. Comparative transcriptome analysis showed that photosynthesis and soluble sugar metabolism were significantly affected in the osnac050 mutant lines, suggesting that OsNAC050 may participate in the above molecular pathways in the response to low temperature stress. The results will enhance our understanding of molecular mechanisms underlying the responses to cold stress in rice and can provide new strategies for engineering cold-tolerance in high-yielding rice varieties.

Keywords

OsNAC050; CRISPR-Cas9; cold stress; RNA-seq; photosynthesis; soluble sugar

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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