Giacomelli, M.; Monti, M.; Pezzola, D.C.; Lonardi, S.; Bugatti, M.; Missale, F.; Cioncada, R.; Melocchi, L.; Giustini, V.; Villanacci, V.; Baronchelli, C.; Manenti, S.; Imberti, L.; Giurisato, E.; Vermi, W. Immuno-Contexture and Immune Checkpoint Molecule Expression in Mismatch Repair Proficient Colorectal Carcinoma. Cancers2023, 15, 3097.
Giacomelli, M.; Monti, M.; Pezzola, D.C.; Lonardi, S.; Bugatti, M.; Missale, F.; Cioncada, R.; Melocchi, L.; Giustini, V.; Villanacci, V.; Baronchelli, C.; Manenti, S.; Imberti, L.; Giurisato, E.; Vermi, W. Immuno-Contexture and Immune Checkpoint Molecule Expression in Mismatch Repair Proficient Colorectal Carcinoma. Cancers 2023, 15, 3097.
Giacomelli, M.; Monti, M.; Pezzola, D.C.; Lonardi, S.; Bugatti, M.; Missale, F.; Cioncada, R.; Melocchi, L.; Giustini, V.; Villanacci, V.; Baronchelli, C.; Manenti, S.; Imberti, L.; Giurisato, E.; Vermi, W. Immuno-Contexture and Immune Checkpoint Molecule Expression in Mismatch Repair Proficient Colorectal Carcinoma. Cancers2023, 15, 3097.
Giacomelli, M.; Monti, M.; Pezzola, D.C.; Lonardi, S.; Bugatti, M.; Missale, F.; Cioncada, R.; Melocchi, L.; Giustini, V.; Villanacci, V.; Baronchelli, C.; Manenti, S.; Imberti, L.; Giurisato, E.; Vermi, W. Immuno-Contexture and Immune Checkpoint Molecule Expression in Mismatch Repair Proficient Colorectal Carcinoma. Cancers 2023, 15, 3097.
Abstract
CRCMSS/pMMR contain a significantly increased fraction of TREM2+ macrophages (TAMs) and CD66+ neutrophils (TANs) together with decrease of CD4-CD8-CD3+ double negative T lymphocytes (DNTs); no differences were revealed by the analysis of myeloid and plasmacytoid dendritic cell populations. A fraction of tumor-infiltrating T-cells display an exhausted phenotype, co-expressing PD-1 and TIM-3. Remarkably, expression of PD-L1 on fresh tumor cells and TAMs was undetectable even after in vitro stimulation with interferon-γ. These findings confirm the immune suppressive microenvironment of CRCMSS/pMMR characterized by dense infiltration of TAMs, occurrence of TANs, lack of DNTs, T-cell exhaustion and interferon-γ unresponsiveness by host and tumor cells. Appropriate bypass strategies should consider these combinations of immune escape mechanisms in CRCMSS/pMMR.
Medicine and Pharmacology, Pathology and Pathobiology
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