Pedrosa, V.A.; Chen, K.; George, T.J.; Fan, Z.H. Gold Nanoparticle-Based Microfluidic Chips for Capture and Detection of Circulating Tumor Cells. Biosensors2023, 13, 706.
Pedrosa, V.A.; Chen, K.; George, T.J.; Fan, Z.H. Gold Nanoparticle-Based Microfluidic Chips for Capture and Detection of Circulating Tumor Cells. Biosensors 2023, 13, 706.
Pedrosa, V.A.; Chen, K.; George, T.J.; Fan, Z.H. Gold Nanoparticle-Based Microfluidic Chips for Capture and Detection of Circulating Tumor Cells. Biosensors2023, 13, 706.
Pedrosa, V.A.; Chen, K.; George, T.J.; Fan, Z.H. Gold Nanoparticle-Based Microfluidic Chips for Capture and Detection of Circulating Tumor Cells. Biosensors 2023, 13, 706.
Abstract
Liquid biopsy has been developed to diagnose and monitor cancer. Despite the recent advances in investigating early cancer detection and diagnosis strategies, there is still a lack of improvements in capturing (CTCs). To archive this, we developed an efficient CTC detection system by integrating gold nanoparticles and a microfluidic platform, which can achieve CTC capture within 120 min. The microdevice was functionalized by immobilizing antibodies specific to the target cells. The method was tested with spiked tumor cells in whole human blood with three pancreatic cancer cell lines and compared with purified cancer cells suspended in a phosphate buffer solution. Our results captured pancreatic cancer cells spiked in blood samples with efficiency (90±3.2)% and were used to isolate CTCs from patients with metastatic pancreatic cancer. This methodology will enable the production of functionalized, patterned surfaces that interact with tumor cells, enhancing the selective capture of CTCs from biological assays.
Chemistry and Materials Science, Analytical Chemistry
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