Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Spontaneous Osteogenic Differentiation of Human Mesenchymal Stem Cells by Tuna Bone-Derived Hydroxyapatite Composites with Green Tea Polyphenol-Reduced Graphene Oxide

Moon Sung Kang
† ORCID logo ,
Rowoon Park
† ,
Hyo Jung Jo
,
Yong Cheol Shin
,
Chang-Seok Kim
ORCID logo ,
Suong-Hyu Hyon
,
Suck Won Hong
* ORCID logo ,
Junghwan Oh
* ORCID logo ,
Dong-Wook Han
* ORCID logo
†
These authors equally contributed to this work.
Version 1 : Received: 2 May 2023 / Approved: 3 May 2023 / Online: 3 May 2023 (09:54:45 CEST)

A peer-reviewed article of this Preprint also exists.

Kang, M.S.; Park, R.; Jo, H.J.; Shin, Y.C.; Kim, C.-S.; Hyon, S.-H.; Hong, S.W.; Oh, J.; Han, D.-W. Spontaneous Osteogenic Differentiation of Human Mesenchymal Stem Cells by Tuna-Bone-Derived Hydroxyapatite Composites with Green Tea Polyphenol-Reduced Graphene Oxide. Cells 2023, 12, 1448. Kang, M.S.; Park, R.; Jo, H.J.; Shin, Y.C.; Kim, C.-S.; Hyon, S.-H.; Hong, S.W.; Oh, J.; Han, D.-W. Spontaneous Osteogenic Differentiation of Human Mesenchymal Stem Cells by Tuna-Bone-Derived Hydroxyapatite Composites with Green Tea Polyphenol-Reduced Graphene Oxide. Cells 2023, 12, 1448.

Abstract

In recent years, bone tissue engineering (BTE) has made significant progress in promoting the direct and functional connection between bone and graft, including osseointegration and osteoconduction, to facilitate the healing of damaged bone tissues. Herein, we introduce a new, environmentally friendly, and cost-effective method for synthesizing reduced graphene oxide (rGO) and hydroxyapatite (HAp). The method uses epigallocatechin-3-O-gallate (EGCG) as a reducing agent to synthesize rGO (E-rGO), and HAp powder is obtained from Atlantic bluefin tuna (Thunnus thynnus). The physicochemical analysis indicated that the E-rGO/HAp composites had exceptional properties for use as BTE scaffolds, as well as high purity. Moreover, we discovered that E-rGO/HAp composites facilitated not only proliferation, but also early and late osteogenic differentiation of human mesenchymal stem cells (hMSCs). Our work suggests that E-rGO/HAp composites may play a significant role in promoting the spontaneous osteogenic differentiation of hMSCs, and we envision that the E-rGO/HAp composites could serve as promising candidates for BTE scaffolds, stem cell differentiation stimulators, and implantable device components due to their biocompatible and bioactive properties. Overall, we suggest a new approach for developing cost-effective and environmentally friendly E-rGO/HAp composite materials for BTE application.

Keywords

bone tissue engineering; hydroxyapatite; reduced graphene oxide; human mesenchymal stem cell; osteogenic differentiation

Subject

Chemistry and Materials Science, Biomaterials

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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