Wang, X.; Memon, S.P.; Panhwar, Q.A.; Memon, S.U.R.; Qasim, M.; Younas, M.U.; Naher, U.A. Biomolecular Characterization of Salt tolerant Plant Growth Promoting Rhizobacteria Isolated from Spinach (Spinacia oleracea L.) Grown in Saline Soil. Preprints2023, 2023050109. https://doi.org/10.20944/preprints202305.0109.v1
APA Style
Wang, X., Memon, S.P., Panhwar, Q.A., Memon, S.U.R., Qasim, M., Younas, M.U., & Naher, U.A. (2023). Biomolecular Characterization of Salt tolerant Plant Growth Promoting Rhizobacteria Isolated from Spinach (Spinacia oleracea L.) Grown in Saline Soil. Preprints. https://doi.org/10.20944/preprints202305.0109.v1
Chicago/Turabian Style
Wang, X., Muhammad Usama Younas and Umme Aminun Naher. 2023 "Biomolecular Characterization of Salt tolerant Plant Growth Promoting Rhizobacteria Isolated from Spinach (Spinacia oleracea L.) Grown in Saline Soil" Preprints. https://doi.org/10.20944/preprints202305.0109.v1
Abstract
Soil salinity is one of the most serious problems for achieving better crop growth and yield. The study was conducted to isolate, characterize and screen salt tolerant microbes from spinach grown in saline soils. The efficient salt tolerant microbes were characterized for beneficial traits such as nitrogen fixation, phosphate solubilization, indoleacetic acid (IAA), biofilm production, catalase and antagonistic activities, salt tolerance and optimization of bacterial fermentation. Molecular identification of potential microbes was done by 16S rRNA gene sequencing. The collected soil samples were moderately to strongly saline, alkaline in nature, low in N and P while adequate in K and Ca concentration. A total of 17 salt tolerant bacteria differing in colony and cell morphology were characterized. All isolated rhizobacterial microbes had capability to biosynthesize phytohormone IAA, 14 were able to fix atmospheric N2, 11 microbes solubilized inorganic P, 10 produced biofilm and 15 were positive in catalase activity. The isolated rhizobacteria showed salt tolerance up to 10 % induced salinity, only six microbes tolerated salt concentration of 12.5% however, none of any rhizobacteria remained viable at 15% NaCl amended media plates. The two potential salt tolerant microbes (NIA SP-6 & NIA SP-11) were screened and found for optimum growth curve and fermentation at pH 7.0 after 48 hrs of incubation period. Both potential microbes were identified as Enterobacter cloacae (NIA SP-06) and Bacillus safensis (NIA SP-11). Hence, it can be concluded that isolated salt tolerant rhizobacterial microbes have potential for beneficial traits and have ability for salt tolerance under saline conditions.
Keywords
Activity; beneficial traits; bacterial count; inoculation; rhizosphere; salinity; salt tolerance
Subject
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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