Version 1
: Received: 2 May 2023 / Approved: 3 May 2023 / Online: 3 May 2023 (02:31:59 CEST)
How to cite:
Shibata, M.; Kitagawa, S.; Unekawa, M.; Takizawa, T.; Nakahara, J. Calcitonin Gene-related Peptide mRNA Synthesis in Trigeminal Ganglion Neurons after Cortical Spreading Depolarization. Preprints2023, 2023050088. https://doi.org/10.20944/preprints202305.0088.v1
Shibata, M.; Kitagawa, S.; Unekawa, M.; Takizawa, T.; Nakahara, J. Calcitonin Gene-related Peptide mRNA Synthesis in Trigeminal Ganglion Neurons after Cortical Spreading Depolarization. Preprints 2023, 2023050088. https://doi.org/10.20944/preprints202305.0088.v1
Shibata, M.; Kitagawa, S.; Unekawa, M.; Takizawa, T.; Nakahara, J. Calcitonin Gene-related Peptide mRNA Synthesis in Trigeminal Ganglion Neurons after Cortical Spreading Depolarization. Preprints2023, 2023050088. https://doi.org/10.20944/preprints202305.0088.v1
APA Style
Shibata, M., Kitagawa, S., Unekawa, M., Takizawa, T., & Nakahara, J. (2023). Calcitonin Gene-related Peptide mRNA Synthesis in Trigeminal Ganglion Neurons after Cortical Spreading Depolarization. Preprints. https://doi.org/10.20944/preprints202305.0088.v1
Chicago/Turabian Style
Shibata, M., Tsubasa Takizawa and Jin Nakahara. 2023 "Calcitonin Gene-related Peptide mRNA Synthesis in Trigeminal Ganglion Neurons after Cortical Spreading Depolarization" Preprints. https://doi.org/10.20944/preprints202305.0088.v1
Abstract
Migraine is a debilitating neurovascular disorder characterized by recurrent headache attacks of moderate to severe intensity. Calcitonin gene-related peptide (GGRP), which is abundantly expressed in trigeminal ganglion (TG) neurons, plays a crucial role in migraine pathogenesis. Cortical spreading depolarization (CSD), the biological correlate of migraine aura, activates the trigeminovascular system. In the present study, we investigated CGRP mRNA expression in TG neurons in a CSD-based mouse migraine model. Our in situ hybridization analysis showed that CGRP mRNA expression was observed in smaller neuronal populations. CSD did not significantly change the density of CGRP mRNA-synthesizing neurons in the ipsilateral TG. However, the cell sizes of CGRP mRNA-synthesizing TG neurons were significantly larger in the 48 h and 72 h post-CSD groups than in the control group. The proportions of CGRP mRNA-synthesizing TG neurons bearing cell diameters less than 14 μm became significantly less at several time points after CSD. In contrast, we found significantly greater proportions of CGRP mRNA-synthesizing TG neurons bearing cell diameters of 14–18 μm at 24 h, 48, and 72 h post-CSD. We deduce that the CSD-induced upward cell size shift in CGRP mRNA-synthesizing TG neurons might be causative of greater disease activity and/or less responsiveness to CGRP-based therapy.
Medicine and Pharmacology, Neuroscience and Neurology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
